Detection of eicosanoids in epiretinal membranes of patients suffering from proliferative vitreoretinal diseases
Detection of eicosanoids in epiretinal membranes of patients suffering from proliferative vitreoretinal diseases
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DOI：
10.1136/bjo.81.1.58
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发表时间：
1997-01-01
影响因子：
4.1
通讯作者：
Spitznas, M
中科院分区：
文献类型：
--
作者：
Augustin, AJ;Grus, FH;Spitznas, M
目标-花生四烯酸通过脂氧合酶代谢为15-HETE（15-羟基二十碳四烯酸）和15-HPETE（15-氢过氧二十碳四烯酸），据信其影响组织培养中的增殖。15-HETE是15-HPETE的还原产物。细胞增殖被认为是由15-HPETE减少和15-HETE增加。本研究的目的是调查这些脂氧合酶的产品的存在下，并比较膜从不同的疾病processes. Methods视网膜前膜的15例患有增生性玻璃体视网膜病变（PVR，n = 7）和增生性糖尿病视网膜病变（PDR; n = 8），在玻璃体切除术和分析的手段，薄层色谱法。结果-15-HETE和15-HPETE均在PVR和PDR患者眼的膜中鉴定，HETE值显著高于HPETE值（HETE/HPETE比5.2）（p < 0.05）。结论-本研究表明，类花生酸存在于PVR和PDR患者的视网膜前膜组织中。考虑到HETE增加细胞增殖而HPETE抑制细胞增殖，可以想象类二十烷酸是促进增殖性视网膜疾病中膜生长调节的额外因素。因此，抑制脂氧合酶可能是这些疾病的治疗方法。
Aim-Arachidonic acid is metabolised via lipoxygenase to 15-HETE (15-hydroxyeicosatetraenoic acid) and 15-HPETE (15-hydroperoxyeicosatetraenoic acid), which are believed to influence proliferation in tissue culture. 15-HETE is the reduction product of 15-HPETE. Cell proliferation is believed to be decreased by 15-HPETE and increased by 15-HETE. The aim of this study was to investigate epiretinal membranes for the presence of these lipoxygenase products and to compare membranes from different disease processes.Methods-Epiretinal membranes of 15 patients suffering from proliferative vitreoretinopathy (PVR, n = 7) and proliferative diabetic retinopathy (PDR; n = 8) were removed during vitrectomy and analysed by means of thin layer chromatography. The plates were evaluated by digital image analysis.Results-Both 15-HETE and 15-HPETE were identified in membranes from eyes of patients with PVR and PDR with HETE values significantly higher (p < 0.05) than HPETE values (HETE/HPETE ratio 5.2).Conclusion-This study demonstrates that eicosanoids are present in the epiretinal membrane tissue of patients with PVR and PDR. Considering that HETE increases cell proliferation while HPETE inhibits it, it is conceivable that eicosanoids are an additional factor contributing to the regulation of membrane growth in proliferative retinal disorders. Thus, inhibition of lipoxygenase could be a therapeutic approach in these diseases.