Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato.
Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato.
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DOI：
10.1111/j.1365-313x.1992.00153.x
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发表时间：
1992-03
期刊：
影响因子：
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通讯作者：
Lifang Shi;Robert T. Gast;Manjula Gopalraj;Neil E. Olszewski
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文献类型：
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作者：
Lifang Shi;Robert T. Gast;Manjula Gopalraj;Neil E. Olszewski
番茄 (Lycopersicon esculentum) 基因 (GAST1) 编码的 RNA 在喷洒 GA3 后，在 GA 缺陷 gib1 突变体的芽中丰度增加了 20 倍以上。治疗后 2 小时即可检测到 GAST1 RNA 水平的增加，并且水平继续增加至少另外 10 小时。处理后 12 至 24 小时之间，GAST1 RNA 的量开始下降，48 小时时的水平几乎与水处理的对照植物相当。核径流分析表明，用 GA3 处理 8 小时后，GAST1 基因的转录仅增加了三倍，表明 GA 在转录和转录后均发挥作用。 ABA 部分抑制 GA 介导的 GAST1 RNA 丰度增加，而乙烯利、激动素和 2,4-D 几乎没有影响。 GAST1 RNA 在未经处理的叶、茎、叶柄和花中可检测到，但在根中却检测不到。 GAST1 基因编码 0.7 kb 的转录本。 GAST1 cDNA 和基因组克隆的序列表明该基因被三个内含子打断，并可能编码一个功能未知的 112 个氨基酸的蛋白质。
A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3 has been characterized. An increase in GAST1 RNA levels is detectable 2 h after treatment and levels continue to increase for at least an additional 10 h. Between 12 and 24 h following treatment, the amount of GAST1 RNA begins to decline and at 48 h the level is nearly equivalent to that of water-treated control plants. Nuclear runoff analysis indicates that 8 h after treatment with GA3, transcription of the GAST1 gene has increased only threefold, suggesting that GA acts both transcriptionally and post-transcriptionally. ABA partially inhibits the GA-mediated increase in GAST1 RNA abundance while ethephon, kinetin, and 2,4-D have little effect. GAST1 RNA is detectable in untreated leaves, stems, petioles and flowers, but not in roots. The GAST1 gene encodes a 0.7 kb transcript. The sequence of the GAST1 cDNA and genomic clones indicates that the gene is interrupted by three introns and potentially encodes a 112 amino acid protein of unknown function.