Biomimetic Synthesis of Lysozyme−Silica Hybrid Hollow Particles Using Sonochemical Treatment: Influence of pH and Lysozyme Concentration on Morphology
Biomimetic Synthesis of Lysozyme−Silica Hybrid Hollow Particles Using Sonochemical Treatment: Influence of pH and Lysozyme Concentration on Morphology
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DOI：
10.1021/cm071011v
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发表时间：
2007-08
影响因子：
8.6
通讯作者：
Toru Shiomi;T. Tsunoda;A. Kawai;and Fujio Mizukami;K. Sakaguchi
中科院分区：
文献类型：
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作者：
Toru Shiomi;T. Tsunoda;A. Kawai;and Fujio Mizukami;K. Sakaguchi
研究了溶菌酶的二氧化硅沉淀活性和控制所得颗粒形态的可能性。溶菌酶不仅可以作为二氧化硅沉淀剂，还可以作为沉淀产物的组分，即形成溶菌酶和二氧化硅的复合物。搅拌产生溶菌酶-二氧化硅混合颗粒状颗粒（L-SHG），而超声化学处理产生溶菌酶-二氧化硅混合中空颗粒（L-SHH）。溶菌酶的二氧化硅沉淀活性和L-SHH的形成仅在pH 9左右发生。在最佳pH条件下，溶菌酶浓度对L-SHH的形貌有影响。与其他碱性蛋白质相比，溶菌酶的发泡性是形成中空结构的关键因素。对L-SHG和L-SHH的形成机理进行了讨论。
The silica precipitation activity of lysozyme and the possibility of controlling the resulting particle morphologies were investigated. Lysozyme can act as not only a silica precipitation agent but also as a component of the precipitation products, namely, forming a composite of lysozyme and silica. Stirring produced lysozyme−silica hybrid granular particles (L-SHGs), while a sonochemical treatment resulted in lysozyme−silica hybrid hollow particles (L-SHHs). The silica precipitation activity of lysozyme and the formation of L-SHHs occur only around pH 9. At the optimized pH, the lysozyme concentration was found to affect the morphologies of the L-SHHs. Compared with other basic proteins, the foaming properties of lysozyme appear to be a key factor in the formation of hollow structures. The proposed formation mechanisms of L-SHGs and L-SHHs are also discussed.