Expression and cell distribution of group I and group II metabotropic glutamate receptor subtypes in Taylor-type focal cortical dysplasia
Expression and cell distribution of group I and group II metabotropic glutamate receptor subtypes in Taylor-type focal cortical dysplasia
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DOI：
10.1046/j.1528-1157.2003.54802.x
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发表时间：
2003-06-01
期刊：
影响因子：
5.6
通讯作者：
Troost, D
中科院分区：
文献类型：
--
作者：
Aronica, E;Gorter, JA;Troost, D
目的：局灶性皮质发育不良(FCD)是难治性癫痫的主要原因之一。FCD致痫的细胞机制(S)仍然很大程度上不清楚。由于最近的研究表明代谢型谷氨酸受体亚型(MGluRs)在癫痫的发生中起作用，我们研究了mGluRs在FCD标本中的表达和细胞分布模式。方法：对15例癫痫手术期间采集的人FCD标本进行了mGluR亚型的免疫细胞化学检测。结果：大多数FCD标本中均有较强的mGluRlα和mGluR5(组mGluRs)免疫反应(IR)。MGluR1a在神经元亚群(主要是大型发育不良细胞)中表达，而mGluR5在发育不良神经元细胞中表达比例较高。II组mGluRs(mGluR2/3)IR的发生率低于I组mGluRs，一般出现在
Purpose: Focal cortical dysplasia (FCD) is known to be a major cause of intractable epilepsy. The cellular mechanism(s) underlying the epileptogenicity of FCD remain largely unknown. Because recent studies indicate that metabotropic glutamate receptor subtypes (mGluRs) play a role in epileptogenesis, we investigated the expression and cellular distribution pattern of mGluRs in FCD specimens.Methods: Immunocytochemical expression of group I and group II mGluR subtypes was investigated in 15 specimens of human FCD obtained during epilepsy surgery.Results: Strong mGluRlalpha and mGluR5 (group I mGluRs) immunoreactivity (IR) was observed in the majority of FCD specimens in dysplastic as well as in heterotopic neurons. mGluRla was expressed in a subpopulation of neurons (mainly large dysplastic cells), whereas mGluR5 was represented in a higher percentage of dysplastic neuronal cells. Group II mGluRs (mGluR2/3) IR was observed less frequently than that in group I mGluRs and generally appeared in