Single-molecule imaging in live bacteria cells.
Single-molecule imaging in live bacteria cells.
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活细菌细胞的单分子成像。
DOI：
10.1098/rstb.2012.0355
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zhang,Shunyuan
中科院分区：
文献类型：
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作者：
Ritchie,Ken;Lill,Yoriko;Sood,Chetan;Lee,Hochan;Zhang,Shunyuan
细菌，例如大肠杆菌和新月柄杆菌，是生物学中研究最多、或许也是了解最透彻的生物体。从这些生物体中获得的对生命系统的理解取得了巨大的进步。由于细菌尺寸小且高度弯曲，单分子技术在细菌中的应用呈现出独特的困难。本次综述的目的是展示过去 10 年来在细菌单分子成像方面取得的进展，并展望未来，在特征良好且可控的模型系统（例如 E.大肠杆菌可以使人们更好地理解通过经典集成方法无法解决的基本生物学问题。
Bacteria, such asEscherichia coliandCaulobacter crescentus, are the most studied and perhaps best-understood organisms in biology. The advances in understanding of living systems gained from these organisms are immense. Application of single-molecule techniques in bacteria have presented unique difficulties owing to their small size and highly curved form. The aim of this review is to show advances made in single-molecule imaging in bacteria over the past 10 years, and to look to the future where the combination of implementing such high-precision techniques in well-characterized and controllable model systems such asE. colicould lead to a greater understanding of fundamental biological questions inaccessible through classic ensemble methods.