Effects of maternal treatment of dehydroepiandrosterone (DHEA) on serum lipid profile and hepatic lipid metabolism-related gene expression in embryonic chickens
Effects of maternal treatment of dehydroepiandrosterone (DHEA) on serum lipid profile and hepatic lipid metabolism-related gene expression in embryonic chickens
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DOI：
10.1016/j.cbpb.2009.12.005
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发表时间：
2010-04-01
影响因子：
2.2
通讯作者：
Zou, Sixiang
中科院分区：
文献类型：
--
作者：
Chen, Juan;Tang, Xue;Zou, Sixiang
在过去的十年中，出现了许多证据表明，母亲在怀孕期间饮食的改变可能会不可逆地影响胎儿的生理和生化功能。为探讨母体日粮中添加脱氢表雄酮（DHEA）对鸡胚脂质代谢的影响，研究了母鸡和鸡胚的血脂谱和肝脏脂质代谢相关基因的表达。将16周龄雏鸡随机分为3组（n = 30），分别在商品日粮中添加0、20和100 mg/kg DHEA。在DHEA处理后收集卵，并在37.5 ℃和60%的相对湿度下孵育。从母鸡和鸡胚中采集血液和肝脏样品。DHEA处理导致母鸡和胚胎鸡体重下降和相对肝脏重量增加，而血液甘油三酯（TG），总胆固醇（TC），低密度脂蛋白-胆固醇（LDL-C）和非酯化脂肪酸（NEFA）的浓度在胚胎发育期间20 mg DHEA/kg组与对照组相比显著降低。孵化时用20 mg DHEA/kg处理后，乙酰辅酶A羧化酶（ACC）和肉毒碱棕榈酰转移酶I（CPTI）基因的表达也减少。然而，血液TC，肝脂肪酸合成酶（FAS）和羟甲基戊二酰辅酶A还原酶（HMGR）基因表达显着上调100 mg DHEA/kg组在胚胎发育和孵化。总体而言，这项研究的结果表明，母亲的饮食治疗与DHEA调节血脂代谢和肝脏基因表达。(C)2010年爱思唯尔公司All rights reserved.
Over the last decade, much evidence emerged to suggest that alterations in maternal diets during pregnancy may irreversibly affect aspects of physiological and biochemical functions in the fetus. To explore the effects of maternal dietary treatments with dehydroepiandrosterone (DHEA) on lipid metabolism in the embryo, we investigated serum lipid profile and hepatic lipid metabolism-related gene expression in the maternal and embryonic chicken. Sixteen-week-old pullets were allocated into 3 groups (n = 30), and after laying, they were provided with a commercial diet supplemented with DHEA at 0, 20 or 100 mg/kg diet. Eggs were collected after DHEA treatment and incubated at 37.5 degrees C and a relative humidity of 60%. Blood and liver samples were collected from hens and embryonic chickens. DHEA treatment resulted in decreased body weight and increased relative liver weight in both maternal and embryonic chickens, while the concentrations of blood triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C) and non-esterified fatty acid (NEFA) were significantly lower in the 20 mg DHEA/kg group as compared to the control group during embryonic development. The expression of acetyl CoA carboxylase (ACC) and carnitine palmitoyl transferase I (CPTI) gene was also reduced following treatment with 20 mg DHEA/kg at hatching. However, blood TC, and hepatic fatty acid synthase (FAS) and hydroxy methylglutaryl-CoA reductase (HMGR) gene expression were significantly up-regulated in the 100 mg DHEA/kg group during embryonic development and hatching. Overall, the results of this study indicate that maternal dietary treatment with DHEA regulates serum lipid metabolism and hepatic gene expression. (C) 2010 Elsevier Inc. All rights reserved.