Effect of Testosterone on TRPV1 Expression in a Model of Orofacial Myositis Pain in the Rat
Effect of Testosterone on TRPV1 Expression in a Model of Orofacial Myositis Pain in the Rat
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睾酮对口面部肌炎疼痛模型大鼠TRPV1表达的影响
DOI：
10.1007/s12031-017-1009-7
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Zhou, Qing
中科院分区：
文献类型：
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作者：
Bai, Xiaofeng;Zhang, Xia;Zhou, Qing
最近的临床研究表明，瞬时受体电位香草酸1（TRPV1）激动剂诱导的疼痛反应存在性别差异。然而，TRPV1相关慢性疼痛中这些差异的机制仍不清楚。在本研究中，我们调查炎症和性腺激素对TRPV1在三叉神经节表达的影响。将完全弗氏佐剂（CFA）注射到大鼠左咬肌中，建立炎性疼痛模型。对CFA注射后雄性和雌性大鼠三叉神经节中TRPV1 mRNA和蛋白水平进行了评估。研究了CFA诱导的三叉神经节TRPV1 mRNA和蛋白表达的变化，这些变化来自睾丸切除（ODX）雄性大鼠和睾酮替代ODX大鼠。此外，TRPV1 mRNA水平在三叉神经节从卵巢切除（OVX）的雌性和ODX雄性大鼠与他莫昔芬治疗进行了评估。我们发现，TRPV1的mRNA和蛋白质的水平在三叉神经节从雌性大鼠CFA注射后显着高于从幼稚雌性大鼠的神经节。CFA诱导的炎性痛觉过敏并没有改变雄性大鼠三叉神经节中TRPV1的表达。炎症开始后第3天，ODX雄性三叉神经节中TRPV 1 mRNA和蛋白表达水平显着上调。然而，CFA诱导的炎性疼痛对睾丸酮替代ODX大鼠中TRPV 1 mRNA或蛋白表达没有显著影响。此外，他莫昔芬不能抑制CFA注射后OVX雌性和ODX雄性大鼠TRPV1表达的上调。总之，这些数据表明TRPV1功能的性别差异可能部分由感觉神经节中的性别依赖性TRPV1表达介导。在该大鼠慢性炎性疼痛模型中，替吉奥在抑制TRPV1表达中起关键作用。
Recent clinical studies have revealed sex differences in response to transient receptor potential vanilloid 1 (TRPV1) agonist-induced pain. However, the mechanism of these differences in TRPV1-related chronic pain remains unclear. In the present study, we investigate the effects of inflammation and gonadal hormones on TRPV1 expression in trigeminal ganglia. Inflammatory pain was modeled by injecting complete Freund's adjuvant (CFA) into the left masseter muscle in rats. TRPV1 mRNA and protein levels in the trigeminal ganglia of male and female rats following CFA injection were assessed. CFA-induced changes in TRPV1 mRNA and protein expression in the trigeminal ganglia from orchidectomized (ODX) male rats and testosterone-replaced ODX rats were examined. Additionally, TRPV1 mRNA levels in the trigeminal ganglia from ovariectomized (OVX) female and ODX male rats treated with tamoxifen were assessed. We found that the levels of TRPV1 mRNA and protein in the trigeminal ganglia from female rats following CFA injection were significantly higher than in the ganglia from naive female rats. CFA-induced inflammatory hyperalgesia did not alter TRPV1 expression in the trigeminal ganglia from male rats. The TRPV1 mRNA and protein expression levels in the ODX male trigeminal ganglia were significantly upregulated on day 3 following the initiation of inflammation. However, CFA-induced inflammatory pain had no significant effect on TRPV1 mRNA or protein expression in testosterone-replaced ODX rats. Furthermore, tamoxifen was unable to inhibit the upregulation of TRPV1 expression in OVX female and ODX male rats after CFA injection. In summary, these data indicate that gender differences in TRPV1 function may be, in part, mediated by sex-dependent TRPV1 expression in sensory ganglia. Testosterone plays a key role in the inhibition of TRPV1 expression in this rat chronic inflammatory pain model.