Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns.
Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns.
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根据 rDNA 分布模式推断西瓜属物种与 Benincaseae 部落（葫芦科）某些属的细胞遗传学关系
DOI：
10.1186/s12862-016-0656-6
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发表时间：
2016-04-18
影响因子：
3.4
通讯作者：
Han YH
中科院分区：
文献类型：
--
作者：
Li KP;Wu YX;Zhao H;Wang Y;Lü XM;Wang JM;Xu Y;Li ZY;Han YH
利用荧光原位杂交(FISH)技术对5S和45S rDNA进行比较定位，是确定近缘种间细胞遗传学关系的有效工具。以栽培西瓜(C.lanatus subsp.)Vergaris)、绵毛委陵菜(C.lanatus subsp.)粘精子属(Colocynthis)和诺氏绒毛虫(C.naudinianus)有2个45S rDNA基因座和1个5S rDNA基因座，其中一个与45S rDNA基因座同线。Ecirrhosus和绵毛委陵菜亚种。羊肚菌有一个45S rDNA基因座和两个5S rDNA基因座，分别位于不同的染色体上。在不同的染色体上分别有一个5S和一个45S rDNA基因座。此外，还研究了5S和45S rDNA在贝纳卡亚族其他属几个物种中的分布。结论本研究证实了栽培西瓜(C.lanatus subsp.)在进化上的亲缘关系。Vergaris)、绵毛委陵菜(C.lanatus subsp.)Mucosospermus和C.colocynthis。我们的结果也支持羊肚菌亚种。羊毛瓜不是栽培西瓜的野生形式，而是一个单独的作物物种。此外，目前的细胞遗传学分析表明，黄瓜与黄瓜的亲缘关系比与西瓜或棘果的亲缘关系更近，但具有独特的位置，可能是连接西瓜和黄瓜的桥梁。
BackgroundComparative mapping of 5S and 45S rDNA by fluorescent in situ hybridization (FISH) technique is an excellent tool to determine cytogenetic relationships among closely related species.ResultsIn this study, the number and position of 5S and 45S rDNA loci in all Citrullus species and subspecies were determined. The cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus, C. colocynthis and C. naudinianus (or Acanthosicyos naudinianus) had two 45S rDNA loci and one 5S rDNA locus which was located syntenic to one of the 45S rDNA loci. C. ecirrhosus and C. lanatus subsp. lanatus had one 45S rDNA locus and two 5S rDNA loci, each located on a different chromosome. C. rehmii had one 5S and one 45S rDNA locus positioned on different chromosomes. The distribution of 5S and 45S rDNA in several species belonging to other genera in Benincaseae tribe was also investigated. The distribution pattern of rDNAs showed a great difference among these species.ConclusionsThe present study confirmed evolutionary closeness among cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus and C. colocynthis. Our result also supported that C. lanatus subsp. lanatus was not a wild form of the cultivated watermelon instead was a separate crop species. In addition, present cytogenetic analysis suggested that A. naudinianus was more closely related to Cucumis than to Citrullus or Acanthosicyos, but with a unique position and may be a link bridge between the Citrullus and the Cucumis.