Immunocytochemical study of PC12 cells grafted to the brain of immature rats.
Immunocytochemical study of PC12 cells grafted to the brain of immature rats.
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移植到未成熟大鼠大脑中的 PC12 细胞的免疫细胞化学研究。
DOI：
10.1007/bf00261353
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发表时间：
1985
影响因子：
2
通讯作者：
Jaeger,CB
中科院分区：
文献类型：
--
作者：
Jaeger,CB
将聚集的PC 12细胞移植到幼年Sprague道利（SD）大鼠的脑中并生长长达两个月。组织化学和免疫细胞化学方法用于鉴定和表征移植的PC 12细胞。发现PC 12细胞在SD大鼠的脑内持续存在并继续增殖，而先前已报道植入外周部位的移植物不增殖。分散和聚集的PC 12细胞移植物含有酪氨酸羟化酶和芳香族氨基酸脱羧酶的免疫反应性，但不表达苯乙醇胺N-甲基转移酶。乙酰胆碱酯酶活性低于组织化学检测水平在所有年龄段的移植。在聚集移植物中，大多数PC 12细胞呈圆形，但也有一些小的PC 12细胞簇形成短突起。在一个月大的移植物中，当位于大脑皮层深层附近时，突起形成的PC 12细胞最为突出。宿主衍生的毛细血管和星形胶质细胞在连续的阶段被并入PC 12聚集移植物中。移植物的血脑屏障不完整。这项研究表明，移植的PC 12细胞具有恢复表现出内源性多巴胺缺乏的大脑功能的潜力，前提是PC 12细胞中的有丝分裂可以被抑制。
Aggregated PC12 cells were transplanted to the brain of infant Sprague Dawley (SD) rats and grown for up to two months. Histochemical and immunocytochemical methods served to identify and characterize the grafted PC12 cells. It was found that PC12 cells persist and continue to proliferate within the brain of SD rats whereas it has previously been reported that grafts placed into peripheral sites do not proliferate. Dispersed and aggregated PC12 cell grafts contained immunoreactivity to tyrosine hydroxylase and aromatic amino acid decarboxylase, but failed to express phenylethanolamine n-methyltransferase. Acetylcholinesterase activity was below histochemical detection levels in grafts of all ages. In aggregate grafts most PC12 cells were round but some small clusters of PC12 cells formed short processes. Process-forming PC12 cells were most prominent when positioned adjacent to deep layers of the cerebral cortex in one month old grafts. Hostderived capillaries and astroglia became incorporated into the PC12 aggregate grafts at successive stages. The blood brain barrier of the grafts was incomplete. This study indicates that grafted PC12 cells have some potential to restore function in brains that exhibit an endogenous dopamine deficiency, providing that mitosis in PC12 cells can be inhibited.