Synthesis of uniformly sized molecularly imprinted polymer-coated silica nanoparticles for selective recognition and enrichment of lysozyme
Synthesis of uniformly sized molecularly imprinted polymer-coated silica nanoparticles for selective recognition and enrichment of lysozyme
复制标题
合成尺寸均匀的分子印迹聚合物涂层二氧化硅纳米粒子，用于选择性识别和富集溶菌酶
DOI：
10.1039/c2jm32734a
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Chen, Guonan
中科院分区：
文献类型：
--
作者：
Lin, Zian;Xia, Zhiwei;Chen, Guonan
将表面印迹技术与纳米材料相结合是克服模板去除和实现大结合容量的有效解决方案。以正硅酸乙酯（TEOS）为前驱体，合成了平均粒径约为400 nm的单分散、尺寸均匀的SiO2纳米粒子（NPs），并通过γ-甲基丙烯酰氧基丙基三甲氧基硅烷（γ-MAPS）的化学修饰将乙烯基引入到SiO2纳米粒子表面。随后，分子印迹聚合物（MIP）涂层共聚和锚定到乙烯基改性的二氧化硅纳米粒子分散在水介质中与溶菌酶（Lyz）作为模板的表面。采用扫描电镜（SEM）、透射电镜（TEM）和傅里叶变换红外光谱（FT-IR）对所得MIP包覆SiO2纳米粒子的形貌和结构进行了表征。详细研究了聚合和吸附条件，以获得最高的选择性和结合容量。在优化条件下，印迹纳米粒子对模板的结合亲和力比非印迹纳米粒子高，相应的印迹因子（a）达到1.68。竞争性实验结果表明，该印迹纳米粒子对模板分子具有较高的选择性。所得的Lyz-MIP二氧化硅纳米粒不仅可以选择性地从混合蛋白质中提取目标蛋白，而且可以特异性地从人血清中富集Lyz。此外，还对印迹纳米粒子的稳定性和再生性能进行了研究，结果表明印迹纳米粒子具有良好的重复使用性。
Combining surface imprinting with nanomaterials is an effective solution to overcome template removal and achieve large binding capacity. In this work, highly monodisperse and uniform-sized silica nanoparticles (NPs) with average diameter of similar to 400 nm were synthesized by using tetraethoxysilane (TEOS) as a single precursor, and then vinyl groups were introduced onto the surface of silica NPs by chemical modification of gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS). Subsequently, the molecularly imprinted polymer (MIP) coating was copolymerized and anchored onto the surface of vinyl modified silica NPs dispersed in aqueous media with lysozyme (Lyz) as a template. The morphology and structure property of the resultant MIP-coated silica NPs were characterized by scanning electronic microscopy (SEM), transmission electronic microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR). The polymerization and adsorption conditions were investigated in detail in order to obtain the highest selectivity and binding capacity. Under the optimized conditions, the imprinted nanoparticles showed higher binding affinity toward the template than non-imprinted (NIP) nanoparticles, and the corresponding imprinted factor (a) reached 1.68. The specificity for Lyz recognition was evaluated with competitive experiments, indicating the imprinted nanoparticles have a higher selectivity for the template. The resultant Lyz-MIP silica NPs could not only selectively extract a target protein from mixed proteins, but also specifically enrich Lyz from human serum. In addition, the stability and regeneration were also investigated, which indicated the imprinted silica NPs had excellent reusability.