X-chromosomal localization of mammalian Y-linked genes in two XO species of the Ryukyu spiny rat
X-chromosomal localization of mammalian Y-linked genes in two XO species of the Ryukyu spiny rat
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DOI：
10.1159/000071608
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发表时间：
2002-01-01
影响因子：
1.7
通讯作者：
Suzuki, H
中科院分区：
文献类型：
--
作者：
Arakawa, Y;Nishida-Umehara, C;Suzuki, H
琉球棘鼠（Tokudaia属）是日本南西诸岛中部特有的一种鼠种，具有独特的染色体核型，染色体数目为奇数，细胞学上无法识别Y染色体。产自Amamioshima的Tokudaia osimensis和产自Tokudaia sp.的染色体数目分别为2n = 25和2n = 45，推测为单一的X染色体。小鼠X探针杂交受损的X染色体，除了在女性标本的短臂的远端部分的T osimensis和在一个男性和一个女性的Tokudaia sp.荧光原位杂交与Tspy（睾丸特异性蛋白，Y-编码）基因从男性和女性细胞Tokudaia sp.通过PCR定位Tspy的远端部分的X染色体的长臂。来自Tokudaia sp.的另一个Y相关基因Zfy也定位于两个物种的同一区域。虽然Sry基因在这个物种中是不存在的，目前的结果表明，Y染色体片段携带功能Y连锁基因，如Tspy和Zfy，易位到远端部分的长臂的X染色体。版权所有（C）2002 S. Karger AG，巴塞尔。
Ryukyu spiny rats (genus Tokudaia), which are endemic to the central part of the Nansei Shoto archipelago in Japan, have unique karyotypes with odd numbers of chromosomes and no cytologically recognizable Y chromosome. The chromosome numbers of Tokudaia osimensis from Amamioshima and of Tokudaia sp. from Tokunoshima are 2n = 25 and 2n = 45, respectively, with a putative single X chromosome. The mouse X probe hybridized to the impaired X chromosome, except for the distal part of the short arm in a female specimen of T osimensis and in one male and one female of Tokudaia sp. Fluorescence in situ hybridization with the Tspy (testis-specific protein, Y-encoded) gene from both male and female cells of Tokudaia sp. by PCR localized Tspy to the distal part of the long arm of the X chromosome. Another Y-related gene, Zfy, from Tokudaia sp. was also localized to the same region in both species. Although the Sry gene is absent in this species, the present results suggest that the Y-chromosome segment carrying functional Y-linked genes, such as Tspy and Zfy, is translocated onto the distal part of the long arm of the X chromosome. Copyright (C) 2002 S. Karger AG, Basel.