A METHOD OF PREPARING PERIPHERAL LEUCOCYTES FOR ELECTRON MICROSCOPY
A METHOD OF PREPARING PERIPHERAL LEUCOCYTES FOR ELECTRON MICROSCOPY
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DOI：
10.1016/s0022-5320(65)80075-2
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发表时间：
1965-01-01
期刊：
影响因子：
--
通讯作者：
ANDERSON, DR
中科院分区：
文献类型：
--
作者：
ANDERSON, DR
Anticoagulated blood is centrifuged until the cells have settled into layers. Without disturbing the buffy coat, all but the last drop of plasma is removed using a Pasteur pipette. Glutaraldehyde fixative is carefully layered over the sedimented cells, again with minimal disruption of the white cell layer. After 15 min. or more, a disk of leucocytes embedded in solidified plasma can be removed with only a thin layer of erythrocytes adhering. The disk is cut into thin slices which are placed in osmium fixative for 1-2 hr. Dehydration and infiltration with embedding medium is carried out as usual. The slices are embedded with an orientation such that sections are cut in the plane of the slice and include consecutive layers of erythrocytes, leukocytes, and platelets. By phase microscopic examination of sections 1 or 2 [mu] thick, the broad-faced block is trimmed to incldue the layer desired in ultrathin sections for electron microscopy. The procedure was de -veloped in order to study leucocytes, but the block could also be trimmed to study the platelet layer.
