TROPHOBLAST LEUKOCYTE-COMMON ANTIGEN IS EXPRESSED BY HUMAN TESTICULAR GERM-CELLS AND APPEARS ON THE SURFACE OF ACROSOME-REACTED SPERM
TROPHOBLAST LEUKOCYTE-COMMON ANTIGEN IS EXPRESSED BY HUMAN TESTICULAR GERM-CELLS AND APPEARS ON THE SURFACE OF ACROSOME-REACTED SPERM
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DOI：
10.1095/biolreprod41.2.285
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发表时间：
1989-08-01
影响因子：
3.6
通讯作者：
JOHNSON, PM
中科院分区：
文献类型：
--
作者：
ANDERSON, DJ;MICHAELSON, JS;JOHNSON, PM
小鼠单克隆抗体H316与人滋养细胞、白细胞、某些上皮细胞和几种恶性细胞类型的细胞表面抗原反应。我们发现H316抗体也识别由减数分裂前和减数分裂后的人类睾丸生殖细胞合成的抗原，并在甲醇固定的睾丸、附睾和射精精子的顶体区域表达。该抗原在新鲜射精的活动精子表面表达较差，但在含有人血清白蛋白（HSA）的培养基中孵育6小时或与钙离子载体A23187孵育60分钟后，在大多数活精子上可检测到（两种处理都会引起精子顶体变化，称为获能和顶体反应）。我们发现，H316识别的抗原从带放射性标记的离子载体处理过的精子提取物中免疫沉淀为单个宽50 kDa，并且hsa激活的精子与该抗体预孵育引起中度但显著的抑制仓鼠卵穿透。这些数据表明，H316单克隆抗体识别的抗原是由睾丸生殖细胞合成的，并在能性/顶体反应的精子群体上表面表达。它作为人类精子顶体反应标志物的潜力，以及在精子-卵子或精子-淋巴细胞相互作用中可能的生物学作用，值得进一步研究。
The murine monoclonal antibody H316 reacts with a cell-surface antigen of human trophoblast, leukocytes, certain epithelia, and several malignant cell types. We have found that the H316 antibody also recognizes an antigen synthesized by pre- and post-meiotic human testicular germ cells and is expressed in the acrosomal region of methanol-fixed testicular, epididymal, and ejaculated sperm. The antigen is poorly expressed on the surface of fresh ejaculated motile sperm, but is detectable on most viable sperm after a 6-h incubation in medium containing human serum albumin (HSA), or 60-min incubation with the calcium ionophore A23187 (both treatments induce sperm acrosomal changes termed capacitation and acrosome reaction). We found that antigen recognized by H316 is immunoprecipitated as a single, broad 50 kDa from band radiolabeled ionophore-treated sperm extracts and that preincubation of HSA-capacitated sperm with this antibody causes a moderate, but significant, inhibition of hamster egg penetration. These data indicate that the antigen recognized by the H316 monoclonal antibody is synthesized by testicular germ cells and is surface-expressed on capacitated/acrosome-reacted sperm populations. Its potential as a human sperm acrosome reaction marker, and possible biological role in sperm-egg or sperm-lymphocyte interactions, warrants further investigation.