Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning （HEGS） System
Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning （HEGS） System
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DOI：
10.1016/s1672-6308(08)60087-6
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发表时间：
2009-12
期刊：
影响因子：
4.8
通讯作者：
A. Shimizu;S. Kawasaki
中科院分区：
文献类型：
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作者：
A. Shimizu;S. Kawasaki
高密度连锁图谱是各种生物性状基因组分析的重要工具。我们开发的紧凑型多凝胶系统HEGS(高效基因组扫描)是一种高通量和高性价比的电泳仪。利用该系统，一个人仅用两个月的时间，就构建了一个高密度(平均间隔2.3 cM)的AFLP图谱和63个SSR标记。所定位的AFLP标记中，超过50%的标记在几个组合中普遍具有多态性，15%的标记适用于水稻旱地类型和低地类型之间的遗传近缘杂交。该系统可用于各种标记的快速分析。
High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS (high efficiency genome scanning) is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density (average interval 2.3 cM) map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.