Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot, Scophthalmus maximus.
Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot, Scophthalmus maximus.
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DOI：
10.1016/j.fsi.2013.07.033
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发表时间：
2013-10
影响因子：
4.7
通讯作者：
Na Wang;Xianli Wang;Changgeng Yang;Song‐lin Chen
中科院分区：
文献类型：
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作者：
Na Wang;Xianli Wang;Changgeng Yang;Song‐lin Chen
信号转导子和转录激活子2（STAT 2）是参与I型干扰素信号转导途径的重要分子。迄今为止，除了大西洋鲑鱼和金鱼之外，鱼类中几乎没有STAT 2同源物。本论文首次从大菱鲆中克隆了STAT 2基因，并对其进行了功能鉴定。该基因全长3206 bp，编码793个氨基酸。系统进化树显示大菱鲆STAT 2蛋白与大西洋鲑的亲缘关系最近。亚细胞分布分析表明，STAT 2主要存在于TK细胞的胞质中，在大菱鲆组织和TK细胞中均呈组成型表达，经鳗弧菌和淋巴囊肿病毒（LCDV）刺激后，STAT 2 mRNA的表达量成倍增加。与高等脊椎动物STAT 2不同，大菱鲆STAT 2的核输出信号（内斯）不存在于C端79个氨基酸，而存在于N端137-312个氨基酸（STAT α结构域）。经Poly（I：C）处理后的大菱鲆STAT 2核转位证明其在TK细胞中具有转录活性。这些结果表明STAT 2可能作为转录因子参与大菱鲆的免疫应答。
Signal transducer and activator of transcription 2 (STAT2) is an important molecule involved in the type I interferon signalling pathway. To date, little STAT2 homologue is available in fish except Atlantic salmon and goldfish. In this paper, STAT2 was firstly cloned and characterized from turbot, a marine flatfish with high economic value. Briefly, turbot STAT2 cDNA is 3206 bp in length encoding a predicted protein of 793 amino acids. The phylogenetic tree shows that turbot STAT2 protein shared the closest relationship with Atlantic salmon. Analysis of subcellular distribution indicates that STAT2 is mainly present in the cytoplasm of TK cells.Stat2mRNA is constitutively expressed in widespread tissues and induced by several folds in turbot tissues and TK cells after stimulation with Vibrio anguillarum and lymphocystis disease virus (LCDV). Unlike the higher vertebrate STAT2, turbot STAT2 nuclear export signal (NES) exists not in the C-terminal 79 amino acids but in N-terminal 137–312 amino acids (STAT_alpha domain). The nuclear translocation of turbot STAT2 after Poly(I:C) treatment proved its transcription activity in TK cells. All these results suggested that STAT2 may be involved in the immune response in turbot as a transcription factor.