Development of Recombinant Arenavirus-Based Vaccines.
Development of Recombinant Arenavirus-Based Vaccines.
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基于重组沙粒病毒的疫苗的开发。
DOI：
10.1007/978-1-4939-6869-5_8
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
delaTorre,JuanCarlos
中科院分区：
文献类型：
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作者：
Martínez-Sobrido,Luis;delaTorre,JuanCarlos
沙粒病毒反向遗传学的发展为沙粒病毒的分子和细胞生物学研究提供了新的、强有力的手段。基于细胞的微型基因组系统的使用已经允许检查参与沙粒病毒复制和转录的顺式和反式作用因子，以及在不需要使用沙粒病毒的活形式的情况下鉴定新的抗沙粒病毒药物靶标。同样，现在可以完全从克隆的cDNA中拯救感染性沙粒病毒，这些cDNA在其基因组中含有预定的突变，以研究病毒-宿主相互作用和发病机制。沙粒病毒遗传学的这些进展也促进了筛选以鉴定抗沙粒病毒药物和追求产生减毒活沙粒病毒疫苗候选物的新策略。此外，表达外源感兴趣基因（GOI）的三节段（r3）沙粒病毒的产生开启了实施基于活减毒沙粒病毒的疫苗载体方法的可能性。在这一章中，我们将总结实施基于质粒的反向遗传学技术的发展r3沙粒病毒表达外来GOI的实施作为疫苗载体。
The development of arenavirus reverse genetics has provided investigators with a novel and powerful approach for the investigation of the arenavirus molecular and cell biology. The use of cell-based minigenome systems has allowed examining thecis- andtrans-acting factors involved in arenavirus replication and transcription, and the identification of novel anti-arenaviral drug targets without requiring the use of live forms of arenaviruses. Likewise, it is now feasible to rescue infectious arenaviruses entirely from cloned cDNAs containing predetermined mutations in their genomes to investigate virus-host interactions and mechanisms of pathogenesis. These advances in arenavirus genetics have also facilitated screens to identify anti-arenaviral drugs and the pursuit of novel strategies to generate live-attenuated arenavirus vaccine candidates. Moreover, the generation of tri-segmented (r3) arenaviruses expressing foreign genes of interest (GOI) has opened the possibility of implementing live-attenuated arenaviruses-based vaccine vector approaches. In this chapter, we will summarize the implementation of plasmid-based reverse genetics techniques for the development of r3 arenaviruses expressing foreign GOI for their implementation as vaccine vectors.