Establishment of a model of cortical bone repair in mice
Establishment of a model of cortical bone repair in mice
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DOI：
10.1007/s00223-002-2120-4
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发表时间：
2003-07-01
影响因子：
4.2
通讯作者：
Mackie, EJ
中科院分区：
文献类型：
--
作者：
Campbell, TM;Wong, WT;Mackie, EJ
已经建立了用于小鼠的皮质骨修复模型。皮质骨缺损由贯穿胫骨骨干整个直径的钻孔组成。最初填充钻孔部位的血肿在损伤后5天内被间充质外观的细胞侵入。到第9天，整个钻孔部位均存在矿化编织骨小梁。邻近钻孔部位的骨膜反应，包括新骨和软骨形成，先于钻孔部位的骨组织沉积。伤后4周造模新生编织骨，髓腔恢复正常，6周皮质结构基本正常。免疫组织化学研究表明，III型胶原在第5天在钻孔部位内表达，在第7天达到峰值，并且在第9天减少。相反。在第7天，在钻孔部位首次检测到I型胶原，到第9天染色更强烈。骨桥蛋白在钻孔部位的表达与该位置新骨的矿化过程一致。这里描述的骨修复模型提供了一种在小鼠中容易识别的位置诱导可再现的骨损伤的方法。这将是有用的，在骨细胞功能的调查，在小鼠品系已受到遗传操作。
A model of cortical bone repair has been established for use in mice. The cortical defect consisted of a hole drilled through the entire diameter of the tibial diaphysis. The hematoma that initially filled the drill site was invaded by cells of mesenchymal appearance within 5 days of injury. Trabeculae of mineralized woven bone were present throughout the drill site by day 9. A reaction in the periosteum adjacent to the drill site, consisting of both new bone and cartilage formation, preceded deposition of bone tissue in the drill site. New woven bone was modeled to restore the marrow cavity to normal by 4 weeks after injury, and almost normal cortical structure was achieved by 6 weeks after injury. lmmunohistochemical studies indicated that type III collagen was expressed within the drill site by day 5, reached a peak at day 7, and was diminished by day 9. In contrast. type I collagen was first detectable in the drill site at day 7, and staining was more intense by day 9. Osteopontin expression in the drill site coincided with the process of mineralization of new bone in this location. The model of bone repair described here provides a method for inducing reproducible bone lesions in a readily identifiable location in mice. It will be useful in the investigation of bone cell function in mouse strains that have been subjected to genetic manipulation.