Generation of viable fish from cryopreserved primordial germ cells
Generation of viable fish from cryopreserved primordial germ cells
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DOI：
10.1002/mrd.20577
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发表时间：
2007-02-01
影响因子：
2.5
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Kobayashi, Terumasa;Takeuchi, Yutaka;Yoshizaki, Goro
越来越多的野生鱼类面临灭绝的危险，这往往是人类活动的结果。配子和胚胎的超低温保存对于濒危物种的保存和恢复具有巨大的潜力。父亲和母亲的遗传信息的保存是必不可少的。然而，尽管这项技术已成功地应用于许多鱼类的精子，但缺乏可靠的方法来长期保存鱼卵和胚胎。在这里，我们描述了一个协议，用于虹鳟鱼（虹鳟鱼）原始生殖细胞（PGCs）和文件的恢复活鱼配子来自这些冷冻保存的祖细胞。生殖嵴（GR），这是胚胎组织含有PGCs，成功地冷冻保存在培养基中含有1.8 M乙二醇（EG）。解冻后的原始生殖细胞移植到同种异体幼鱼的腹腔中，在受体性腺中分化为成熟的精子和卵子。此外，冷冻保存的精子与冷冻保存的原始生殖细胞的卵受精，导致了可育F1鱼的发育。这种PGC冷冻保存技术是一种很有前途的工具，努力拯救受威胁的鱼类。此外，这种方法具有很大的潜力，保持驯化的鱼类品种携带有商业价值的特性，用于水产养殖目的。
An increasing number of wild fish species are in danger of extinction, often as a result of human activities. The cryopreservation of gametes and embryos has great potential for maintaining and restoring threatened species. The conservation of both paternal and maternal genetic information is essential. However, although this technique has been successfully applied to the spermatozoa of many fish species, reliable methods are lacking for the long-term preservation of fish eggs and embryos. Here, we describe a protocol for use with rainbow trout (Oncorhynchus mykiss) primordial germ cells (PGCs) and document the restoration of live fish from gametes derived from these cryopreserved progenitors. Genital ridges (GRs), which are embryonic tissues containing PGCs, were successfully cryopreserved in a medium containing 1.8 M ethylene glycol (EG). The thawed PGCs that were transplanted into the peritoneal cavities of allogenic trout hatchlings differentiated into mature spermatozoa and eggs in the recipient gonads. Furthermore, the fertilization of eggs derived from cryopreserved PGCs by cryopreserved spermatozoa resulted in the development of fertile F1 fish. This PGC cryopreservation technique represents a promising tool in efforts to save threatened fish species. Moreover, this approach has significant potential for maintaining domesticated fish strains carrying commercially valuable traits for aquaculture purposes.