HUMAN-ANTIBODY RESPONSES TO OUTER ENVELOPE ANTIGENS OF PORPHYROMONAS-GINGIVALIS SEROTYPES
HUMAN-ANTIBODY RESPONSES TO OUTER ENVELOPE ANTIGENS OF PORPHYROMONAS-GINGIVALIS SEROTYPES
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DOI：
10.1111/j.1600-0765.1995.tb01247.x
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发表时间：
1995-01-01
影响因子：
3.5
通讯作者：
STEFFEN, MJ
中科院分区：
文献类型：
--
作者：
EBERSOLE, JL;STEFFEN, MJ
检查牙龈卟啉单胞菌主要抗原成分同质性的免疫学研究提出了 3 种血清型，并表明血清型在个体患者中的分布有限。这些研究促使我们定义免疫显性抗原和针对牙龈卟啉单胞菌血清型的免疫反应的分布。在本研究中，当针对 A 型牙龈卟啉单胞菌（即 33277）进行测试时，牙周炎患者的血清 IgG 抗体水平最常高于正常受试者。近 1/3 的患者表现出针对牙龈卟啉单胞菌多种血清型的抗体显着升高，这显然是由交叉反应抗原引起的。我们确定了从三种血清型获得的外膜蛋白和抗原模式之间的显着差异。此外，结果发现患者对特定血清型的定性和定量抗原反应模式有相当大的相似性。 IgG 抗体水平 (ELISA) 与免疫印迹中测定的总反应水平之间存在很强的正相关性，并且与特定抗原的抗体比例也存在正相关性。这些发现表明对这些抗原的反应构成对完整微生物的反应的主要部分。此外，针对特定抗原带的抗体的检测表明对每种牙龈卟啉单胞菌血清型的早期反应。我们的研究结果表明，牙周炎患者的一个亚群通常对牙龈卟啉单胞菌的多种血清型产生广泛的血清抗体反应，并且可能定义患有牙龈卟啉单胞菌疾病的患者群体。最后，我们的结果表明牙龈卟啉单胞菌的抗原成分更加一致，这可能会增强免疫干扰这种微生物引起的疾病的策略的潜力。
Immunological studies examining the homogeneity of the major antigenic components of P. gingivalis have suggested 3 serotypes and have indicated a limited distribution of the serotypes in an individual patient. These studies prompted us to define the immunodominant antigens and distribution of immune responses to P. gingivalis serotypes. Serum IgG antibody levels in periodontitis patients in the present study were most frequently elevated above the normal subjects when tested against P. gingivalis serotype A (i.e., 33277). Nearly 1/3 of the patients showed significantly elevated antibody to multiple serotypes of the P. gingivalis apparently resulting from cross-reacting antigens. We determined distinctive differences among outer envelope protein and antigen patterns obtained from the three serotypes. Moreover, the results identified considerable similarities in the qualitative and quantitative antigen response patterns among patients to a particular serotype. There was a strong positive correlation between IgG antibody levels (ELISA) and the total level of reactivity determined in the immunoblots, as well as a positive correlation to the proportion of antibody to particular antigens. These findings suggest that responses to these antigens comprised a major portion of the response to the intact microorganism. Additionally, the detection of antibody to particular antigen bands was indicative of early responses to each of the P. gingivalis serotypes. The results of our study indicate that a subpopulation of periodontitis patients develop an extensive serum antibody response often to multiple serotypes of PI gingivalis and may define a patient population with a P. gingivalis disease. Finally our results indicate a more consistent antigenic composition for P. gingivalis which may enhance the potential for strategies to immunologically interfere with disease caused by this microorganism.