Independent transcription of miR-281 in the intron of ODA in Drosophila melanogaster
Independent transcription of miR-281 in the intron of ODA in Drosophila melanogaster
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果蝇 ODA 内含子中 miR-281 的独立转录
DOI：
10.1016/j.bbrc.2008.12.010
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发表时间：
2009-01-23
影响因子：
3.1
通讯作者：
Li, Fei
中科院分区：
文献类型：
--
作者：
Xiong, Huiping;Qian, Jinjun;Li, Fei
MicroRNA (miRNA) 最近因其在转录后调控中的作用而受到广泛关注。然而，miRNA转录的研究滞后于基因克隆和功能分析的研究。果蝇中的 MiR-281 位于鸟氨酸脱羧酶抗酶 (ODA) 基因同工型 RA 的第一个内含子中。由于转录起始位点 (TSS) 不同，ODA 具有三种同种型。表达谱分析表明 miR-281 不与任何 ODA 亚型共表达。我们使用 RACE 技术扩增了 miR-281 的初级转录本。 pri-miRNA 为 2149 by，具有聚 (A) 尾和规范的聚腺苷酸化信号 (AATAAA)。染色质免疫沉淀分析证实了低磷酸化的 Pol-II 和转录因子 Myc 在核心 miR-281 启动子区域的结合。 miR-281 的丰度与任何 ODA 同工型的表达均不呈正相关或负相关，表明 ODA 对 miR-281 的转录影响很小。 (C) 2008 Elsevier Inc. 保留所有权利。
MicroRNAs (miRNAs) have recently received much interest for their role in post-transcriptional regulation. However, the study of miRNA transcription lags behind that of gene cloning and functional analysis. The MiR-281 in Drosophila melanogaster is located in the first intron of isoform RA of the ornithine decarboxylase antizyme (ODA) gene. ODA has three isoforms because of alternative transcription start sites (TSSs). Expression profile analysis indicated that miR-281 is not co-expressed with any ODA isoform. We amplified the primary transcripts of miR-281 using the RACE technique. The pri-miRNA is 2149 by with a poly (A) tail and a canonical polyadenylation signal (AATAAA). Chromatin immunoprecipitation analysis confirmed the binding of hypophosphorylated Pol-II and the transcription factor Myc at the core miR-281 promoter region. The abundance of miR-281 does not correlate, either positively or negatively, with the expression of any ODA isoform, indicating that ODA has little influence on the transcription of miR-281. (C) 2008 Elsevier Inc. All rights reserved.