Functional analysis on immune deficiency (IMD) homolog gene in Chinese shrimp Fenneropenaeus chinensis
Functional analysis on immune deficiency (IMD) homolog gene in Chinese shrimp Fenneropenaeus chinensis
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中国明对虾免疫缺陷（IMD）同源基因的功能分析
DOI：
10.1007/s11033-013-2988-2
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发表时间：
2014-03-01
影响因子：
2.8
通讯作者：
Li, Fuhua
中科院分区：
文献类型：
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作者：
Feng, Ningning;Wang, Dongdong;Li, Fuhua
IMD通路是调节无脊椎动物先天免疫的重要信号通路。 IMD被认为是连接细胞外信号和细胞内信号的关键适配器。在本研究中，从中国明对虾中分离出编码IMD同源物（FcIMD）的全长cDNA。它由 767 bp 和 483 bp 开放阅读框组成，编码 160 个氨基酸。预测了死亡域，这对于 IMD 的免疫功能至关重要。组织表达分析显示FcIMD mRNA在不同组织中有不同的表达水平。虾体内FcIMD的转录表达在弧菌或微球菌刺激后的早期（1小时）显着诱导，这表明其在虾对细菌感染的免疫中发挥重要作用。当 FcIMD 通过 dsRNA 干扰沉默时，虾抗菌肽 (AMP) 的表达受到影响。通过分析弧菌注射后1小时FcIMD沉默虾中FcRelish、FcDorsal和FcJNK的表达，我们推断IMD参与Relish调节的信号通路，而不参与Dorsal或JNK调节的信号通路。
IMD pathway is an important signaling pathway in regulating the innate immunity of invertebrate. IMD is regarded as a key adaptor to link the extracellular signals and intracellular signals. In the present study, a full-length cDNA encoding an IMD homolog (FcIMD) was isolated from Chinese shrimp Fenneropenaeus chinensis. It consists of 767 bp with a 483 bp open reading frame encoding 160 amino acids. A death domain was predicted which is crucial to the immune function of IMD. Tissue expression analysis showed that FcIMD mRNA had different expression level in different tissues. The transcriptional expression of FcIMD in shrimp was significantly induced at early stage (1 h) after stimulation by Vibrio or Micrococcus which suggested its important role in the immunity of shrimp to bacterium infection. The expressions of shrimp antimicrobial peptides (AMPs) were affected when FcIMD was silenced by dsRNA interference. Through analyzing the expression of FcRelish, FcDorsal and FcJNK in FcIMD silenced shrimp at 1 h post Vibrio injection, we deduced that IMD was involved in Relish regulated signaling pathway, not in Dorsal or JNK regulated signaling pathway.