Low temperature polarized absorption microspectroscopy of single crystals of the reaction center from Rhodobacter sphaeroides wild type strain 2.4.1.
Low temperature polarized absorption microspectroscopy of single crystals of the reaction center from Rhodobacter sphaeroides wild type strain 2.4.1.
复制标题
球形红杆菌野生型菌株2.4.1反应中心单晶的低温偏振吸收显微光谱。
DOI：
10.1111/j.1751-1097.1991.tb02000.x
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发表时间：
1991
影响因子：
3.3
通讯作者：
Parot,PH
中科院分区：
文献类型：
--
作者：
Frank,HA;Aldema,ML;Violette,CA;Parot,PH
在85 K温度下，测定了从球孢杆菌野生型菌株2.4.1分离得到的反应中心配合物的单晶吸光度和偏振吸光度。通过气相扩散技术得到了该配合物的晶体。晶体的光谱实验是使用光学显微光谱仪进行的，该光谱仪具有定制的液态N2流动冷级，其细节在此给出。这些数据证明了在低温下对光合色素-蛋白质复合物单晶进行光谱实验的可行性。
The absorbance and polarized absorbance spectra of single crystals of the reaction center complex isolated fromRhodobacter sphaeroideswild type strain 2.4.1 have been measured at 85 K. The crystals of the complex were obtained by the vapor diffusion technique. The spectroscopic experiments on the crystals were performed using an optical microspectrometer featuring a custom‐built, liquid N2‐flowing cold stage, the details of which are presented herein. These data demonstrate the feasibility of conducting optical spectroscopic experiments at cryogenic temperatures on single crystals of photosynthetic pigment‐protein complexes.