Long-Term Cultured Human Skin Mast Cells Are Suitable for Pharmacological Studies of Anti-Allergic Drugs Due to High Responsiveness to FcεRI Cross-Linking
Long-Term Cultured Human Skin Mast Cells Are Suitable for Pharmacological Studies of Anti-Allergic Drugs Due to High Responsiveness to FcεRI Cross-Linking
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DOI：
10.1271/bbb.100745
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发表时间：
2011-02-01
影响因子：
1.6
通讯作者：
Zuberbier, Torsten
中科院分区：
文献类型：
--
作者：
Guhl, Sven;Artuc, Metin;Zuberbier, Torsten
人皮肤肥大细胞在IL-4+SCF存在下增殖(在8周内扩增18倍)，并对高亲和力IgE受体(Fc Epsilon RI)交联获得深刻的反应性，释放约75%的组胺。在一项概念验证中，我们发现这些细胞对药理学测试很有用。甚至可以看到对脱颗粒的细微抑制。这个模型可能会在新型抗过敏药物的测试中被证明是有价值的。
Human skin mast cells proliferated in the presence of interleukin (IL)-4+SCF (expanding 18-fold in 8 weeks) and acquired profound responsiveness towards high affinity IgE receptor (Fc epsilon RI) cross-linking, liberating about 75% of their histamine. In a proof-of-concept, we found that these cells are useful for pharmacological testing. Even a subtle inhibition of degranulation can be visualized. This model might prove valuable in tests of novel anti-allergic drugs.