Comparative Assessment of SSR and AFLP Markers for Evaluation of Genetic Diversity and Conservation of Fig, Ficus carica L., Genetic Resources in Tunisia
Comparative Assessment of SSR and AFLP Markers for Evaluation of Genetic Diversity and Conservation of Fig, Ficus carica L., Genetic Resources in Tunisia
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DOI：
10.1007/s11105-010-0217-x
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发表时间：
2011-03-01
影响因子：
2.1
通讯作者：
Hannachi, Amel Salhi
中科院分区：
文献类型：
--
作者：
Baraket, Ghada;Chatti, Khaled;Hannachi, Amel Salhi
本研究利用简单序列重复(SSR)和扩增片段长度多态性(AFLP)标记对无花果品种的遗传变异进行了研究。比较了这两种技术在检测突尼斯无花果品种间的变异和建立亲缘关系方面的效率和实用性。我们的结果表明，使用这两个标记系统，突尼斯无花果种质的特点是在脱氧核糖核酸水平上具有较大的遗传多样性，因为大多数AFLP条带都被检测到，并且所有的SSR标记都是多态的。事实上，AFLP和SSR分别检测到351条(342条多态)和57条(57条多态)条带。SSR标记的多态程度最高，平均多态信息含量为0.94，AFLP标记的有效复制率最高(56.9)，标记指数最高(45.2)。AFLP标记的有效标记指数最高(4.19)，SSR标记的有效标记指数最低(0.70)。我们的结果表明：(1)SSR和AFLP片段的独立和联合聚类分析表明，品种之间的聚类与其地理来源、园艺分类和树木性别无关；(2)分子方差分析允许遗传变异在无花果组内和组间的划分，并显示出较大的组内差异；(3)AFLP和SSR标记数据集呈正相关。本研究表明，SSR和AFLP标记可用于品种的遗传多样性分析和品种指纹分析。了解突尼斯甘蓝的遗传多样性和种群结构也可以为该物种的保护和管理提供洞察力。
This study characterises the genetic variability of fig, Ficus carica L., using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. It compares the efficiency and utility of the two techniques in detecting variation and establishing genetic relationships among Tunisian fig cultivars. Our results show that using both marker systems, the Tunisian fig germ plasm is characterised by having a large genetic diversity at the deoxyribonucleic acid level, as most of AFLP bands were detected and all SSR markers were polymorphic. In fact, 351 (342 polymorphic) and 57 (57 polymorphic) bands were detected using AFLP and SSR primers, respectively. SSR markers were the most polymorphic with an average polymorphic information content value of 0.94, while AFLP markers showed the highest effective multiplex ratio (56.9) and marker index (45.2). The effective marker index was recorded highest (4.19) for AFLP markers and lowest (0.70) for the SSR ones. Our results demonstrate that (1) independent as well as combined analyses of cluster analyses of SSR and AFLP fragments showed that cultivars are clustered independently from their geographical origin, horticultural classifications and tree sex; (2) the analysis of molecular variance allowed the partitioning of genetic variation within and among fig groups and showed greater variation within groups and (3) AFLP and SSR markers datasets showed positive correlation. This study suggests the SSR and AFLP markers are suitable for diversity analysis and cultivars fingerprinting. An understanding of the genetic diversity and population structure of F. carica in Tunisia can also provide insight into the conservation and management of this species.