Testicular cell lines
Testicular cell lines
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DOI：
10.1016/j.mce.2003.05.001
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发表时间：
2004-12
影响因子：
4.1
通讯作者：
N. Rahman;I. Huhtaniemi
中科院分区：
文献类型：
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作者：
N. Rahman;I. Huhtaniemi
目前可用的体内或体外永生化细胞系的范围为研究正常和病理细胞功能提供了各种模型系统。这些细胞系来自自发或实验诱导的肿瘤，或通过体外永生化。转基因(TG)技术提供了一种强大的方法，通过癌基因或其他促肿瘤基因的组织特异性表达，可以生产包括恶性肿瘤在内的各种疾病的体内动物模型。TG技术还可以通过将所需的基因插入特定的细胞类型来生产具有特定特征的细胞系，然后这些基因可以在细胞培养中永生化。使用温度敏感的永生化基因提供了控制基因表达的另一个优势，包括要永生的细胞的增殖和分化。关于男性生殖系统，目前有一些睾丸体细胞的细胞系。本文主要介绍睾丸间质细胞和支持细胞的永生化细胞系，特别是用于研究睾丸内分泌功能和肿瘤发生的小鼠细胞系。这些细胞系也为研究激素作用和睾丸细胞相互作用的分子基础提供了有用的工具。
The range of in vivo or in vitro immortalized cell lines currently available provides a variety of model systems for studies of normal and pathological cell functions. The cell lines have been derived from spontaneous or experimentally induced tumors, or through in vitro immortalization. The transgenic (TG) techniques provide a powerful approach, allowing the production of in vivo animal models for a variety of diseases, including malignant tumors, through tissue-specific expression of oncogenes or other tumor-promoting genes. The TG techniques also enable the production of cell lines with specific characteristics, through insertion of desired genes into specific cell types, which can then be immortalized upon cell culture. The use of temperature-sensitive immortalizing genes offers an additional advantage of controlling gene expression, including the proliferation and differentiation of the cells to be immortalized. As regards the male reproductive system, a number of cell lines of testicular somatic cells are currently available. This review covers mainly the immortalized cell lines of testicular Leydig and Sertoli cells, with special reference to murine cell lines for the study of testicular endocrine function and tumorigenesis. These cell lines also provide useful tools to investigate the molecular basis of hormone actions and testicular cell interactions.