Quantifying the effect of pore size and surface treatment on epidermal incorporation into percutaneously implanted sphere-templated porous biomaterials in mice.
Quantifying the effect of pore size and surface treatment on epidermal incorporation into percutaneously implanted sphere-templated porous biomaterials in mice.
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DOI：
10.1002/jbm.a.33125
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发表时间：
2011-09-15
期刊：
影响因子：
--
通讯作者：
Fleckman P
中科院分区：
文献类型：
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作者：
Underwood RA;Usui ML;Zhao G;Hauch KD;Takeno MM;Ratner BD;Marshall AJ;Shi X;Olerud JE;Fleckman P
皮肤和经皮医疗器械之间的窦通常是感染的入口。表皮整合到优化的多孔生物材料中可以密封这个窦。在这项研究中，我们测量了经皮植入小鼠体内的上皮向内生长到球模板多孔聚（2-羟乙基甲基丙烯酸酯）[聚（HEMA）]棒中的情况。该棒含有球形 20、40 或 60μm 孔，经过或未经过表面改性。植入后 3、7 和 14 天，利用 Pankeratin 免疫组织化学和图像分析测量上皮迁移。我们的全球结果显示平均角质形成细胞迁移距离为 81μm +/- 16.85μm (SD)。与 40 和 60μm 孔（分别为 87.04μm +/- 13.38 和 86.63μm +/- 8.31）相比，通过 20μm 孔的迁移时间更短（69.32μm +/- 21.73）。在不考虑孔径和愈合持续时间因素的情况下，迁移不受 CDI 表面改性的影响。上皮整合迅速发生，显示 3 天后平均迁移距离为 74.13μm +/- 12.54，随着时间的推移没有显着进展。这些数据表明，表皮在 3 天内关闭窦，迁移到生物材料中（平均为杆总直径的 11%）并停止。这个过程形成了一个完整的上皮环，没有有袋化或迁移的证据。
The sinus between skin and a percutaneous medical device is often a portal for infection. Epidermal integration into an optimized porous biomaterial could seal this sinus. In this study we measured epithelial ingrowth into rods of sphere-templated porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] implanted percutaneously in mice. The rods contained spherical 20, 40 or 60μm pores with and without surface modification. Epithelial migration was measured 3, 7 and 14 days post-implantation utilizing immunohistochemistry for pankeratins and image analysis. Our global results showed average keratinocyte migration distances of 81μm +/− 16.85μm (SD). Migration was shorter through 20μm pores (69.32μm +/− 21.73) compared to 40 and 60μm (87.04μm +/− 13.38 and 86.63μm +/− 8.31 respectively). Migration was unaffected by CDI surface modification without considering factors of pore size and healing duration. Epithelial integration occurred quickly showing an average migration distance of 74.13μm +/− 12.54 after 3 days without significant progression over time. These data show that the epidermis closes the sinus within 3 days, migrates into the biomaterial (an average of 11% of total rod diameter) and stops. This process forms an integrated epithelial collar without evidence of marsupialization or permigration.