(-)-Anonaine Induces DNA Damage and Inhibits Growth and Migration of Human Lung Carcinoma H1299 Cells
(-)-Anonaine Induces DNA Damage and Inhibits Growth and Migration of Human Lung Carcinoma H1299 Cells
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DOI：
10.1021/jf103488j
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发表时间：
2011-03-23
影响因子：
6.1
通讯作者：
Wang, Hui-Min
中科院分区：
文献类型：
--
作者：
Chen, Bing-Hung;Chang, Hsueh-Wei;Wang, Hui-Min
本研究探讨了（-）-阿诺碱的抗癌作用。在50-200 μ M的浓度范围内，（-）-阿诺卡因在24小时对人肺癌H1299细胞的细胞生长和迁移活性表现出显著的抑制作用，尽管细胞周期分析表明，在上述浓度范围内的（-）-阿诺卡因没有引起细胞周期分布的任何显著变化。H1299细胞的显着的核损伤，观察到10 - 200 μ M（-）-anonaine治疗在彗星试验，而更高浓度（6和30 mM）的（-）-anonaine浓度需要在体外质粒裂解试验中引起DNA损伤。总之，我们的研究结果表明，（-）-阿诺卡因H1299细胞表现出剂量依赖性的抗增殖，抗迁移，和DNA损伤的影响。我们推测，（-）-阿诺碱可以引起细胞周期阻滞和DNA损伤，阻碍癌细胞在72小时的生理行为，因此，它可以作为一个潜在的草药补充剂，用于人类肺癌的化学预防。
The anticancer effects of (-)-anonaine were investigated in this current study. (-)-Anonaine at concentration ranges of 50-200 mu M exhibited significant inhibition to cell growth and migration activities on human lung cancer H1299 cells at 24 h, albeit cell cycle analyses showed that (-)-anonaine at the above concentration ranges did not cause any significant changes in cell cycle distributions. Significant nuclear damages of H1299 cells were observed with 10 200 mu M (-)-anonaine treatment in a comet assay, whereas higher concentrations (6 and 30 mM) of (-)-anonaine concentrations were required to cause DNA damages in an in vitro plasmid cleavage assay. In summary, our results demonstrated that (-)-anonaine exhibited dose dependent antiproliferatory, antimigratory, and DNA-damaging effects on H1299 cells. We inferred that (-)-anonaine can cause cell-cycle arrest and DNA damage to hamper the physiological behavior of cancer cells at 72 h, and therefore, it can be useful as one of the potential herbal supplements for chemoprevention of human lung cancer.