Direct and Protein-Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond
Direct and Protein-Mediated Cell Attachment on Differently Terminated Nanocrystalline Diamond
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DOI：
10.1002/cvde.200906828
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发表时间：
2010-03-01
影响因子：
--
通讯作者：
Lechleitner, Thomas
中科院分区：
文献类型：
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作者：
Klauser, Frederik;Hermann, Martin;Lechleitner, Thomas
本研究的目的是调查直接和蛋白质介导的细胞表面相互作用的润湿性的影响。选择纳米金刚石（NCD）作为细胞生长基底，因为它提供了调整表面特性（如粗糙度或化学终止）的可能性，从而实现亲水或疏水表面。使用两种不同的细胞系（LLC-PK 1和PANC-1）在具有确定的粗糙度但具有不同表面化学性质（氢、氟和氧终止）的NCD膜上研究细胞粘附。在生长培养基中加入和不加入胎牛血清（FBS）的情况下培养它们，以区分直接和介导的细胞粘附。对于这两种细胞系，在不向生长培养基中添加FBS的情况下，细胞不能粘附到疏水表面，而在存在FBS的情况下，它们可以粘附。亲水性表面使得在向培养基中添加和不添加FBS的情况下细胞能够附着。
The aim of this study is to investigate the influence of wettability on direct and protein-mediated cell-surface interactions. Nanocrystalline diamond (NCD) is chosen as a cell growth substrate, because it offers the possibility to adjust surface properties like roughness or chemical termination, thus realizing hydrophilic or hydrophobic surfaces. Cell adhesion is studied on NCD films with a defined roughness but different surface chemistries (hydrogen, fluorine, and oxygen terminations) using two different cell lines (LLC-PK1 and PANC-1). They are cultured with and without the addition of fetal bovine serum (FBS) to the growth medium in order to discriminate between direct and mediated cell adhesion. For both cell lines, the cells are not able to adhere to hydrophobic surfaces without the addition of FBS to the growth medium, whereas they can attach in the presence of FBS. Hydrophilic surfaces enable cell attachment with and without the addition of FBS to the medium.