Reference range of hemolysis index in serum and lithium-heparin plasma measured with two analytical platforms in a population of unselected outpatients
Reference range of hemolysis index in serum and lithium-heparin plasma measured with two analytical platforms in a population of unselected outpatients
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DOI：
10.1016/j.cca.2013.12.010
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发表时间：
2014-02-15
影响因子：
5
通讯作者：
Salvagno, Gian Luca
中科院分区：
文献类型：
--
作者：
Lippi, Giuseppe;Giavarina, Davide;Salvagno, Gian Luca
背景：溶血指数（HI）现已在多种实验室分析仪中提供，但对于抑制测试结果的阈值、不同仪器之间的标准化程度以及不同生物基质中不同参考范围的使用仍然存在疑问。因此，本研究计划使用两个分析平台，在未选择的门诊患者群体中建立血清和肝素锂血浆中 HI 的参考范围。 材料和方法：我们分析了从 135 名未选择的门诊患者中收集的血清和肝素锂样本中的 HI，并根据临床和实验室标准研究所 (CLSI) 的建议定义了相对参考范围。专家护士在早上使用直针静脉穿刺采集样本。使用罗氏 Cobas c501 和西门子 Dimension Vista 1500 评估血清和肝素锂血浆中的 HI。结果：使用 Cobas c501 测量时，血清中无细胞血红蛋白的中位浓度显着高于肝素锂血浆，但使用 Dimension Vista 1500 则不然。根据无细胞血红蛋白阈值对值进行分类后，仪器之间的一致性为 0.75 (p <血清为 0.01)，肝素锂血浆为 0.95 (p < 0.01)。根据 CLSI 文件 C28-A3 计算的血清中罗氏 Cobas c501 的上限为 0.22 g/L，西门子 Dimension Vista 1500 的上限为 025 g/L，而肝素锂血浆中 Cobas c501 的上限为 0.13 g/L，Dimension Vista 1500 的上限为 0.10 g/L。结论：根据我们的数据，不同的无细胞阈值应在血清和肝素锂血浆之间使用血红蛋白来监测放血实践。 (C) 2013 Elsevier B.V. 保留所有权利。
Background: The hemolysis index (HI) is now available in Several laboratory analyzers, but doubts remain about the thresholds for suppressing test results, the degree of standardization among different instrumentations and the use of different reference ranges in different biological matrices. This study was hence planned to establish the reference ranges of HI in serum and lithium-heparin plasma in a population of unselected outpatients, using two analytical platforms.Materials and methods: We analyzed the HI in serum and lithium-heparin samples collected from 135 unselected outpatients, and we also defined the relative reference ranges according to Clinical and Laboratory Standards Institute (CLSI) recommendations. Samples were collected in the morning by expert nurses, using straight needle venipuncture. The HI in serum and lithium-heparin plasma was assessed with Roche Cobas c501 and Siemens Dimension Vista 1500.Results: The median concentration of cell-free hemoglobin was significantly higher in serum than in lithium-heparin plasma when measured with Cobas c501, but not with Dimension Vista 1500. After categorizing values according to cell-free hemoglobin thresholds, the agreement between instruments was 0.75 (p < 0.01) for serum and 0.95 (p < 0.01) for lithium-heparin plasma. The upper limits calculated according to CLSI document C28-A3 were 0.22 g/L for Roche Cobas c501 and 025 g/L for Siemens Dimension Vista 1500 in serum, whereas they were 0.13 g/L for Cobas c501 and 0.10 g/L for Dimension Vista 1500 in lithium-heparin plasma.Conclusions: According to our data, different thresholds of cell-free hemoglobin should be used between serum and lithium-heparin plasma for monitoring phlebotomy practice. (C) 2013 Elsevier B.V. All rights reserved.