SOYBEAN GH3 PROMOTER CONTAINS MULTIPLE AUXIN-INDUCIBLE ELEMENTS
SOYBEAN GH3 PROMOTER CONTAINS MULTIPLE AUXIN-INDUCIBLE ELEMENTS
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DOI：
10.1105/tpc.6.5.645
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发表时间：
1994-05-01
期刊：
影响因子：
11.6
通讯作者：
GUILFOYLE, TJ
中科院分区：
文献类型：
--
作者：
LIU, ZB;ULMASOV, T;GUILFOYLE, TJ
大豆GH 3基因在施用生长素后几分钟内在多种组织和器官中被转录诱导。为了确定赋予生长素诱导的GH 3启动子的序列元件，我们使用凝胶迁移率变动分析，甲基化干扰，缺失分析，接头扫描，定点诱变，和获得的功能分析与最小的花椰菜花叶病毒35 S启动子。我们确定了生长素诱导的GH 3启动子内的至少三个序列元件，并且可以彼此独立地起作用。这些元件中的两个被发现在一个76-bp的片段，这些由两个独立的25-和32-bp的生长素诱导元件。这两个25-和32-bp的生长素诱导型元件都含有恰好位于AATAAG上游的序列TGTCTC。一个额外的生长素诱导元件被发现上游的76-bp的生长素诱导的片段，这可以独立的76-bp的片段的功能。启动子中的两个TGA-box或Hex-like元件（TGACGTAA和TGACGTGGC）是植物核提取物中蛋白质的强结合位点，也可以提高GH 3启动子的生长素诱导水平。GH 3启动子内的多个生长素诱导元件对用该启动子观察到的生长素诱导的总体水平有递增的贡献。
The soybean GH3 gene is transcriptionally induced in a wide variety of tissues and organs within minutes after auxin application. To determine the sequence elements that confer auxin inducibility to the GH3 promoter, we used gel mobility shift assays, methylation interference, deletion analysis, linker scanning, site-directed mutagenesis, and gain-of-function analysis with a minimal cauliflower mosaic virus 35S promoter. We identified at least three sequence elements within the GH3 promoter that are auxin inducible and can function independently of one another. Two of these elements are found in a 76-bp fragment, and these consist of two independent 25- and 32-bp auxin-inducible elements. Both of these 25- and 32-bp auxin-inducible elements contain the sequence TGTCTC just upstream of an AATAAG. An additional auxin-inducible element was found upstream of the 76-bp auxin-inducible fragment; this can function independently of the 76-bp fragment. Two TGA-box or Hex-like elements (TGACGTAA and TGACGTGGC) in the promoter, which are strong binding sites for proteins in plant nuclear extracts, may also elevate the level of auxin inducibility of the GH3 promoter. The multiple auxin-inducible elements within the GH3 promoter contribute incrementally to the overall level of auxin induction observed with this promoter.