Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge.
Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge.
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DOI：
10.1099/ijs.0.63430-0
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发表时间：
2005-03
影响因子：
2.8
通讯作者：
James W. Voordeckers;V. Starovoytov;C. Vetriani
中科院分区：
文献类型：
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作者：
James W. Voordeckers;V. Starovoytov;C. Vetriani
从大西洋中脊36 ° 14' N 33 ° 54' W的一个活跃深海热液喷口烟囱壁上分离到一种嗜热、厌氧、化能自养细菌，命名为TB-2（T）菌株。细胞为革兰氏阴性杆菌，长约1.5 μ m，宽约0.75 μ m。菌株TB-2（T）在45 - 70 ℃（最适55 ℃）、10 - 40 g NaCl l（-1）（最适30 g l（-1））和pH 4.5 - 7.5（最适pH 5.5）之间生长。在最佳条件下的世代时间为50分钟。生长发生在化能自养条件下，以H（2）为能源和CO（2）为碳源。硝酸盐或硫被用作电子受体，分别产生铵和硫化氢。氧、硫代硫酸盐、亚硫酸盐、硒酸盐和砷酸盐不用作电子受体。乙酸盐、乳酸盐、甲酸盐和蛋白胨的存在抑制了生长。基因组DNA的G+C含量为25.6mol%。16 S rRNA基因序列的系统发育分析表明，该微生物与Caminibacter hydrogeniphilus和Caminibacter profundus密切相关（分别为95.9%和96.3%）。基于系统发育、生理和遗传学的考虑，提出该生物代表Caminibacter属中的一个新种，Caminibacter mediatlanticus sp. nov.。模式菌株为TB-2（T）（=DSM 16658（T）=JCM 12641（T））。
A thermophilic, anaerobic, chemolithoautotrophic bacterium, designated strain TB-2(T), was isolated from the walls of an active deep-sea hydrothermal vent chimney on the Mid-Atlantic Ridge at 36 degrees 14' N 33 degrees 54' W. The cells were Gram-negative rods approximately 1.5 microm in length and 0.75 microm in width. Strain TB-2(T) grew between 45 and 70 degrees C (optimum 55 degrees C), 10 and 40 g NaCl l(-1) (optimum 30 g l(-1)) and pH 4.5 and 7.5 (optimum pH 5.5). Generation time under optimal conditions was 50 min. Growth occurred under chemolithoautotrophic conditions with H(2) as the energy source and CO(2) as the carbon source. Nitrate or sulfur was used as the electron acceptor, with resulting production of ammonium and hydrogen sulfide, respectively. Oxygen, thiosulfate, sulfite, selenate and arsenate were not used as electron acceptors. Growth was inhibited by the presence of acetate, lactate, formate and peptone. The G+C content of the genomic DNA was 25.6 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Caminibacter hydrogeniphilus and Caminibacter profundus (95.9 and 96.3 % similarity, respectively). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the genus Caminibacter, Caminibacter mediatlanticus sp. nov. The type strain is TB-2(T) (=DSM 16658(T)=JCM 12641(T)).