THE TERMINUS REGION OF THE ESCHERICHIA-COLI CHROMOSOME CONTAINS 2 SEPARATE LOCI THAT EXHIBIT POLAR INHIBITION OF REPLICATION
THE TERMINUS REGION OF THE ESCHERICHIA-COLI CHROMOSOME CONTAINS 2 SEPARATE LOCI THAT EXHIBIT POLAR INHIBITION OF REPLICATION
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DOI：
10.1073/pnas.84.7.1754
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发表时间：
1987-04-01
影响因子：
11.1
通讯作者：
KUEMPEL, PL
中科院分区：
文献类型：
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作者：
HILL, TM;HENSON, JM;KUEMPEL, PL
大肠杆菌染色体的末端区域包含两个独立的位点，称为 T1 和 T2，抑制复制叉。 T1位于28.5分钟附近，与trpp相邻，T2位于终点区域对面的34.5-35.7分钟，靠近manA。这些位点以极性方式起作用，相对于遗传图谱顺时针方向移动的复制叉在通过 T1 时不会受到抑制，但在 T2 时会受到抑制。类似地，逆时针分叉在 T2 时不被抑制，但在 T1 时被重新抑制。因此，分叉在穿过终点区域并即将离开之前不会受到抑制。对缺失菌株的研究已将 T2 定位在 58 千碱基间隔内，对应于末端区域物理图上的千碱基坐标 387-445。
The terminus region of the chromosome of Escherichia coli contains two separate sites, called T1 and T2, that inhibit replication forks. T1 is located near 28.5 min, which is adjacent to trpp, and T2 is located at 34.5-35.7 min on the opposite side of the terminus region, near manA. The sites act in a polar fashion, and replication forks traveling in a clockwise direction with respect to the genetic map are not inhibited as they pass through T1 but are inhibited t T2. Similarly, counterclockwise forks are not inhibited at T2 but re inhibited at T1. Consequently, forks are not inhibited until they have passed through the terminus region and are about to leave it. Studies with deletion strains have located T2 within a 58-kilobase interval, which corresponds to kilobase coordinates 387-445 on the physical map of the terminus region.