Circular Dichroism of Peptides
Circular Dichroism of Peptides
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DOI：
10.1007/978-1-62703-673-3_17
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Middaugh, C. Russell
中科院分区：
文献类型：
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作者：
Bakshi, Kunal;Liyanage, Mangala R.;Middaugh, C. Russell
圆二色性测量左旋和右旋圆偏振光吸光度的差异，可用于监测肽的二级结构（远紫外）和较大多肽的三级结构（近紫外）。该技术对于螺旋-螺旋转变和结构改变的其他方面特别有用。来自几种低分辨率光谱技术（包括 CD）的数据可以组合起来生成肽结构随环境条件变化的整体图景。
Circular dichroism measures the difference between the absorbance of left-and right-handed circularly polarized light, and can be used to monitor the secondary structure of peptides (far UV) and the tertiary structure of larger polypeptides (near UV). This technique is especially useful for helix-coil transitions and other aspects of structural alterations. Data from several low-resolution spectroscopic techniques, including CD, can be combined to generate an overall picture of peptide structure as a function of environmental conditions.