Tomographic digital subtraction angiography for lung perfusion estimation in rodents
Tomographic digital subtraction angiography for lung perfusion estimation in rodents
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DOI：
10.1118/1.2717384
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发表时间：
2007-05-01
期刊：
影响因子：
3.8
通讯作者：
Johnson, G. Allan
中科院分区：
文献类型：
--
作者：
Badea, Cristian T.;Hedlund, Laurence W.;Johnson, G. Allan
由于对高空间和时间分辨率的综合要求，灌注的体内测量对现有的小动物成像技术如磁共振显微镜、微型计算机断层扫描、微型正电子发射断层扫描和microSPECT提出了挑战。我们证明了使用断层数字减影血管造影（TDSA）的估计灌注在小动物。TDSA通过使用断层合成算法提供三维空间信息来增强传统的数字减影血管造影术（DSA）。TDSA基于一种新颖的范式，即可以以一系列不同的旋转角度连续注射μ L体积的对比剂来再现相同的时间密度曲线。在大鼠肺灌注的研究中建立了TDSA的能力。使用内部开发的成像系统，我们获得了四维（4D）成像的时间分辨率为140毫秒，在100 μ m的平面空间分辨率，和切片厚度毫米的顺序的数据。基于结构化的实验方法，我们优化了TDSA成像，在切片厚度、注射次数、噪声对比度和对伪影的免疫力之间提供了良好的权衡。DSA和TDSA图像均用于创建灌注参数图。TDSA成像在许多领域具有潜在的应用，其中4D中的功能灌注测量可以提供对疾病的动物模型和对治疗的反应的有价值的洞察。(c)2007年美国医学物理学家协会。
In vivo measurements of perfusion present a challenge to existing small animal imaging techniques such as magnetic resonance microscopy, micro computed tomography, micro positron emission tomography, and microSPECT, due to combined requirements for high spatial and temporal resolution. We demonstrate the use of tomographic digital subtraction angiography (TDSA) for estimation of perfusion in small animals. TDSA augments conventional digital subtraction angiography (DSA) by providing three-dimensional spatial information using tomosynthesis algorithms. TDSA is based on the novel paradigm that the same time density curves can be reproduced in a number of consecutive injections of mu L volumes of contrast at a series of different angles of rotation. The capabilities of TDSA are established in studies on lung perfusion in rats. Using an imaging system developed in-house, we acquired data for four-dimensional (4D) imaging with temporal resolution of 140 ms, in-plane spatial resolution of 100 mu m, and slice thickness on the order of millimeters. Based on a structured experimental approach, we optimized TDSA imaging providing a good trade-off between slice thickness, the number of injections, contrast to noise, and immunity to artifacts. Both DSA and TDSA images were used to create parametric maps of perfusion. TDSA imaging has potential application in a number of areas where functional perfusion measurements in 4D can provide valuable insight into animal models of disease and response to therapeutics. (c) 2007 American Association of Physicists in Medicine.