NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation
NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation
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DOI：
10.1016/j.dnarep.2007.02.009
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发表时间：
2007-09-01
期刊：
影响因子：
3.8
通讯作者：
Doherty, Aidan J.
中科院分区：
文献类型：
--
作者：
Pitcher, Robert S.;Green, Andrew J.;Doherty, Aidan J.
利用DNA修复突变体（Delta recA、Delta ku、Delta ligD、Delta ku/ligD、Delta recA/ku/ligD）在耻垢分枝杆菌中检测了非同源末端连接（NHEJ）途径在DNA双链断裂（DSB）修复中的生理作用。野生型和突变型菌株在其生命周期的特定点暴露于一系列剂量的电离辐射。NHEJ突变株（Δ kU、Δ ligD、Δ ku/ligD）在稳定期对电离辐射（IR）的敏感性显著高于野生型M.恶臭然而，在对数生长期，NHEJ突变株和野生型菌株的IR敏感性差异不大。同样，NHEJ突变株比野生型M.恶臭Δ recA突变株在稳定期和对数期对干燥和IR更敏感，但在稳定期对IR的敏感性显著低于Δ recA/ku/ligD三突变株。这些数据表明，NHEJ和同源重组是M.分别在稳定期和对数期内进行smegalone（C）2007 Elsevier B. V.保留所有权利。
The physiological role of the non-homologous end-joining (NHEJ) pathway in the repair of DNA double-strand breaks (DSBs) was examined in Mycobacterium smegmatis using DNA repair mutants (Delta recA, Delta ku, Delta ligD, Delta ku/ligD, Delta recA/ku/ligD). Wild-type and mutant strains were exposed to a range of doses of ionizing radiation at specific points in their life-cycle. NHEJ-mutant strains (Delta kU, Delta ligD, Delta ku/ligD) were significantly more sensitive to ionizing radiation (IR) during stationary phase than wild-type M. smegmatis. However, there was little difference in IR sensitivity between NHEJ-mutant and wild-type strains in logarithmic phase. Similarly, NHEJ-mutant strains were more sensitive to prolonged desiccation than wild-type M. smegmatis. A Delta recA mutant strain was more sensitive to desiccation and IR during both stationary and especially in logarithmic phase, compared to wild-type strain, but it was significantly less sensitive to IR than the Delta recA/ku/ligD triple mutant during stationary phase. These data suggest that NHEJ and homologous recombination are the preferred DSB repair pathways employed by M. smegmatis during stationary and logarithmic phases, respectively (C) 2007 Elsevier B.V. All rights reserved.