Large-scale early in vitro response to actinobacillus actinomycetemcomitans suggests superantigenic activation of T-cells.
Large-scale early in vitro response to actinobacillus actinomycetemcomitans suggests superantigenic activation of T-cells.
复制标题
对伴放线放线杆菌的大规模早期体外反应表明 T 细胞的超抗原激活。
DOI：
10.1177/00220345010800011101
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发表时间：
2001
影响因子：
7.6
通讯作者：
Park,K
中科院分区：
文献类型：
--
作者：
Zadeh,HH;Nalbant,A;Park,K
T细胞对伴生放线杆菌的反应模式在很大程度上是未知的。本研究试图探讨一种假说，即伴生放线菌表达超抗原，能够激活非抗原特异性的T细胞。为此，用伴生放线菌刺激外周血单核细胞，用流式细胞仪检测T细胞早期活化标志物CD69的表达。结果表明，伴生放线杆菌能激活大量的T细胞，其数量与葡萄球菌肠毒素超抗原的数量相近。放线菌伴生菌优先激活表达Vβ5.1和Vβ8的T细胞，而胞外制剂激活Vβ5.1+，Vβ8+和Vβ12+T细胞。在自体和异种抗原提呈细胞存在的情况下，观察到T细胞对伴生放线菌的反应，表明这是一种MHC非限制性反应。因此，伴随放线杆菌的体外反应的特征是大规模的T细胞以Vβ特异性和MHC非限制性的方式激活，这与超抗原的参与是一致的。
The mode of T-cell response to Actinobacillus actinomycetemcomitans is largely unknown. The present study sought to investigate the hypothesis that A. actinomycetemcomitans expresses superantigens, capable of antigen-non-specific T-cell activation. To that end, peripheral blood mononuclear cells were stimulated with A. actinomycetemcomitans, and T-cell expression of the early activation marker, CD69, was determined by flow cytometry. Results showed that A. actinomycetemcomitans activated a large number of T-cells with magnitude similar to that of staphylococcal enterotoxin superantigens. A. actinomycetemcomitans sonicate preferentially activated T-cells expressing Vβ5.1 and Vβ8, while the extracellular preparation activated Vβ5.1+, Vβ8+, and Vβ12+T-cells. T-cell response to A. actinomycetemcomitans was observed in the presence of autologous, as well as heterologous, antigen-presenting cells, suggesting a MHC-non-restricted response. Thus, the in vitro response to A. actinomycetemcomitans is characterized by large-scale T-cell activation in a Vβ-specific and MHC-non-restricted manner, consistent with the involvement of superantigens.