Expression of the OsPI1 gene, cloned from rice roots using cDNA microarray, rapidly responds to phosphorus status
Expression of the OsPI1 gene, cloned from rice roots using cDNA microarray, rapidly responds to phosphorus status
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DOI：
10.1046/j.1469-8137.2003.00748.x
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发表时间：
2003-05
期刊：
影响因子：
9.4
通讯作者：
J. Wasaki;Ryoma Yonetani;T. Shinano;M. Kai;M. Osaki
中科院分区：
文献类型：
--
作者：
J. Wasaki;Ryoma Yonetani;T. Shinano;M. Kai;M. Osaki
植物已经发展了几种适应低磷（P）条件的方法。然而，响应植物磷状况的基因表达系统的调节细节尚不清楚。通过基因芯片技术分离到一个新的磷限制诱导基因OsPI 1（Oryza sativa Phosphate-limitation Inducible Gene 1）。通过北方印迹和定量真实的时间PCR检测mRNA积累。·OsPI 1基因在芽和根中被磷饥饿快速诱导。当磷酸盐供应到缺磷植物，OsPI 1转录迅速消失。OsPI 1 cDNA全长375 bp，含有多个开放阅读框（ORF）。OsPI 1基因与TPSI 1/Mt4家族（磷酸饥饿诱导的新基因家族）具有相同的特性。·OsPI 1可能是一种核糖核酸调节因子，即在磷酸盐饥饿状态下与其他分子结合并调节它们的功能。
Summary • Plants have developed several methods of adapting to conditions of low phosphorus (P). However, details of the regulation of the gene expression system that responds to P status of plants is unknown. Here, a phosphorus limitation inducible novel gene was isolated and characterized to provide further information of plant adaptation to low P. • Rice plants (Oryza sativa) were grown hydroponically with or without P. A novel gene was isolated by cDNA microarray analysis and designated as OsPI1 (Oryza sativaPhosphate-limitation Inducible Gene 1). mRNA accumulation was examined by Northern blot and quantitative real time PCR. • The OsPI1 gene was rapidly induced by phosphate starvation in both shoots and roots. When phosphate was supplied to phosphate-deficient plants, the OsPI1 transcripts rapidly disappeared. OsPI1 cDNA consisted of 375 bp and contained several small open reading frames (ORFs). The OsPI1 gene shows the same characteristics as the TPSI1/Mt4 family (the phosphate starvation inducible novel gene family). • It is suggested that OsPI1 acts as riboregulator, that is, it binds with other molecules under phosphate starvation and regulates their function.