Membrane-mediated inhibition of corticosterone on the release of arginine vasopressin from rat hypothalamic slices
Membrane-mediated inhibition of corticosterone on the release of arginine vasopressin from rat hypothalamic slices
复制标题
膜介导的皮质酮对大鼠下丘脑切片精氨酸加压素释放的抑制
DOI：
10.1016/0006-8993(95)01068-8
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Yi
中科院分区：
文献类型：
--
作者：
Xiu Liu;Yi
用放射免疫法测定了含室旁核和视上核的大鼠下丘脑脑片在静态微室内孵育后精氨酸加压素（AVP）的释放。研究了牛血清白蛋白结合皮质酮（B-BSA）对AVP释放的影响。结果表明：（1）B-BSA在10 - 7 ~ 10 - 4 mol/l浓度范围内，20 min内明显抑制AVP释放，并呈剂量依赖性; (2)RU 38486（10 - 4-10 - 3 mol/L）可部分阻断B-BSA的抑制作用，但其本身不影响AVP的释放。(3)随着培养液中Ca ~（2+）浓度的升高，AVP释放量增加，B-BSA的抑制作用增强;在无Ca ~（2+）时，AVP释放量减少，B-BSA的抑制作用减弱。(4)B-BSA的抑制作用在新霉素的存在下增强，而新霉素本身对AVP的释放没有影响。这些结果表明，皮质酮的抑制作用是快速的，是膜介导的，是非基因组的，而不是经典的基因组，细胞外Ca ~（2+）在这种快速的抑制作用中起作用。
The arginine vasopressin (AVP) released from the hypothalamic slices containing paraventricular and supraoptic nuclei of Sprague-Dawley rats sectioned with vibratome and incubated in static microchambers was measured by radioimmunoassay. The effect of bovine serum albumin-conjugated corticosterone (B-BSA) on the AVP release was investigated. The results were as follows: (1) B-BSA, within 20 min, significantly inhibited AVP release in a dose-dependent manner from 10−7to 10−4mol/l. (2) RU38486 (10−4–10−3mol/I) could partially block the inhibitory effect of B-BSA although it by itself did not change the AVP release. (3) With the elevation of Ca2+concentration in the incubation medium, the AVP release was increased and the inhibitory effect of B-BSA enhanced; while in the absence of Ca2+, the AVP release decreased and the effect of B-BSA attenuated. (4) The inhibitory effect of B-BSA was enhanced in the presence of neomycin which itself had no influence on AVP release. These results indicated that the inhibitory effect of corticosterone is rapid and membrane-mediated which is non-genomic rather than classical genomic, and that the extracellular Ca2+play a role in this rapid inhibitory effect.
类固醇 17-α-雌二醇对肾上腺儿茶酚胺释放的膜介导作用。
DOI：
--
发表时间：
1991
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Lopez,MG;Abad,F;Sancho,C;dePascual,R;Borges,R;Maroto,R;Dixon,W;Garcia,AG
通讯作者：
Garcia,AG