Dithiobissuccinimidyl propionate self assembled monolayer based cholesterol biosensor
Dithiobissuccinimidyl propionate self assembled monolayer based cholesterol biosensor
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DOI：
10.1039/b707000d
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发表时间：
2007-01-01
期刊：
影响因子：
4.2
通讯作者：
Malhotra, Bansi D.
中科院分区：
文献类型：
--
作者：
Arya, Sunil K.;Pandey, Pratibha;Malhotra, Bansi D.
在金（Au）表面制备了二硫代双琥珀酰亚胺丙酸酯（DTSP）自组装膜（SAM），并将其用于胆固醇氧化酶（ChOx）和胆固醇酯酶（ChEt）的共价固定。这些ChOx-ChEt/DTSP/Au生物电极已分别使用电化学阻抗和循环伏安（CV）技术进行了表征。微分脉冲伏安法（DPV）已被用于酶法测定固定化的ChOx和ChEt到DTSP改性的金表面作为胆固醇油酸酯浓度的函数。在ChOx-ChEt/DTSP/Au生物电极上进行的响应测量显示米氏常数（Km）值为0.95 mM，表明酶（ChOx和ChEt）的亲和力增强。ChOx-ChEt/DTSP/Au生物电极在油酸胆固醇浓度为50 ~ 400 mg dl（-1）范围内呈线性关系，在4 ℃下储存时，其保质期超过50天。该生物传感电极的相关系数为0.9973，回归标准偏差为0.859 mA。
A dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) prepared onto a gold (Au) surface has been utilized for covalent immobilization of cholesterol oxidase (ChOx) and cholesterol esterase (ChEt). These ChOx-ChEt/DTSP/Au bio-electrodes have been characterized using electrochemical impedance and cyclic voltammetric (CV) techniques, respectively. Differential pulse voltammetry (DPV) has been used for enzymatic assay of immobilized ChOx and ChEt onto the DTSP modified gold surface as a function of cholesterol oleate concentration. The response measurement conducted on ChOx-ChEt/DTSP/Au bio-electrode reveal the value of Michaelis-Menten constant ( Km) as 0.95 mM suggesting enhanced affinity of enzymes (ChOx and ChEt). The ChOx-ChEt/DTSP/Au bio-electrodes show linearity in range of 50 to 400 mg dl(-1) of cholesterol oleate and the shelf-life of more than 50 days when stored at 4 degrees C. This biosensing electrode shows correlation coefficient of 0.9973 and standard deviation of regression as 0.859 mA.