Immunohistochemical localization of basic fibroblast growth factor in ependymal cells of the rat lateral and third ventricles.
Immunohistochemical localization of basic fibroblast growth factor in ependymal cells of the rat lateral and third ventricles.
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大鼠侧脑室和第三脑室室管膜细胞中碱性成纤维细胞生长因子的免疫组织化学定位。
DOI：
10.1159/000147140
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Fernando Carceller
中科院分区：
文献类型：
--
作者：
Pedro Martinez Cuevas;Guillermo Giménez;Ricardo Martinez;Fernando Carceller
碱性成纤维细胞生长因子（bFGF）的存在下，在第三和侧脑室室管膜的大鼠进行了研究，在光学显微镜下使用多克隆抗体对bFGF，通过未标记的过氧化物酶-抗过氧化物酶的程序; bFGF-免疫反应（bFGF-IR）观察到在整个室管膜细胞层的心室。第三脑室壁下部和底部的室管膜延伸细胞也显示bFGF-IR。延伸细胞突起与下丘脑毛细血管紧密接触。本研究强烈表明，脑室管膜bFGF发挥未知的作用无关，其血管生成或促有丝分裂的能力。
The presence of basic fibroblast growth factor (bFGF) in the third and the lateral ventricular ependyma of the rat has been investigated under the light microscope using a polyclonal antibody against bFGF, through the unlabelled peroxidase-antiperoxidase procedure; bFGF-immunoreactivity (bFGF-IR) is observed in the entire ependymal cell layer of ventricles. Also ependymal tanycytes within the inferior portion of the wall and floor of the third ventricle show bFGF-IR. Tanycytic processes are in close contact with hypothalamic capillaries. The present study strongly suggests that brain ependymal bFGF plays unidentified roles unrelated to its angiogenic or mitogenic capacities.