Mutations induced by DNA lesions in hot spots of the c-Ha-ras gene.
Mutations induced by DNA lesions in hot spots of the c-Ha-ras gene.
复制标题
c-Ha-ras 基因热点 DNA 损伤引起的突变。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Hideo Inoue
中科院分区：
文献类型：
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作者：
H. Kamiya;H. Miura;M. Suzuki;N. Murata;H. Ishikawa;M. Shimizu;Y. Komatsu;T. Murata;T. Sasaki;Hideo Inoue
为了研究几种DNA损伤(O6-甲基鸟嘌呤、8-羟基鸟嘌呤、黄嘌呤、碱基类似物和次黄嘌呤)是否激活c-Ha-ras基因，并确定DNA损伤引起的突变类型，采用DNA盒突变技术将它们导入合成的c-Ha-ras基因。将修饰基因导入小鼠NIH3T3细胞，并对转化细胞中的c-Ha-ras基因进行分析。O6-甲基鸟嘌呤和黄嘌呤诱导突变为A，次黄嘌呤诱导突变为G-8-羟基鸟嘌呤，碱基类似物在修饰和邻近位置引起随机突变。这些结果表明，人工合成的c-Ha-ras基因在检测DNA损伤引起的突变方面是非常有用的。
In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. The modified genes were transfected into mouse NIH3T3 cells and the c-Ha-ras genes present in transformed cells were analysed. O6-methylguanine and xanthine induced a mutation to A, hypoxanthine induced a mutation to G. 8-hydroxyguanine and the abasic site analogue caused random mutations in the modified and adjacent positions. These results indicated that the synthetic c-Ha-ras gene is very useful for the detection of mutations caused by a DNA lesion.