Large-scale isolation of mouse thymic epithelial cells
Large-scale isolation of mouse thymic epithelial cells
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小鼠胸腺上皮细胞的大规模分离
DOI：
10.1007/978-1-0716-2740-2_11
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发表时间：
2023
影响因子：
--
通讯作者：
Takahama Y.
中科院分区：
文献类型：
--
作者：
Ohigashi I;Matsuda-Lennikov M;Takahama Y.
胸腺分为皮质和髓质。皮质和髓质胸腺上皮细胞（TECs）的特征T细胞产生和T细胞选择功能的皮质和髓质微环境中的胸腺。胸腺的酶消化和TEC及其亚群的流式细胞术分离可用于TEC的分子和细胞表征。然而，从小鼠胸腺分离的cTEC和mTEC的细胞结构是有限的。在这一章中，我们描述了分离大量的TEC使用扩大小鼠胸腺，使TEC亚群的生化和蛋白质组学分析的方法。
The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.