A low resolution model for the chromatin core particle by neutron scattering.
A low resolution model for the chromatin core particle by neutron scattering.
复制标题
通过中子散射建立染色质核心颗粒的低分辨率模型。
DOI：
10.1093/nar/4.11.3769
复制
发表时间：
1977
影响因子：
14.9
通讯作者：
E. Bradbury
中科院分区：
文献类型：
--
作者：
P. Suau;G. Kneale;G. W. Braddock;J. Baldwin;E. Bradbury
中子散射研究已被应用到染色质核心颗粒在溶液中，使用对比度变化技术。的回转半径和径向分布函数的对比度依赖性的基础上，它示出的核心颗粒由一个核心包含大部分的组蛋白周围缠绕的DNA螺旋，以下的平均半径为4.5 nm的路径，与一小部分的组蛋白。将形状与内部结构分离，然后进行模型计算，结果表明颗粒的整体形状为尺寸约为100 μ m的扁平圆柱体。11x11x6纳米。DNA精确折叠的进一步细节无法从数据中推导出来，但详细的模型计算支持来自晶体学研究的并行结果（25）。
Neutron scattering studies have been applied to chromatin core particles in solution, using the contrast variation technique. On the basis of the contrast dependance of the radius of gyration and the radial distribution function it is shown that the core particle consists of a core containing most of the histone around which is wound the DNA helix,following a path with a mean radius of 4.5 nm,in association with a small proportion of the histones. Separation of the shape from the internal structure, followed by model calculations shows that the overall shape of the particle is that of a flat cylinder with dimensions ca. 11x11x6 nm. Further details of the precise folding of the DNA cannot be deduced from the data, but detailed model calculations support concurrent results from crystallographic studies(25).Images