Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer
Physical and transcript map of the region between D6S264 and D6S149 on chromosome 6q27, the minimal region of allele loss in sporadic epithelial ovarian cancer
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DOI：
10.1038/sj.onc.1205067
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发表时间：
2002-01-17
期刊：
影响因子：
8
通讯作者：
Ganesan, TS
中科院分区：
文献类型：
--
作者：
Liu, Y;Emilion, G;Ganesan, TS
我们之前已经证明卵巢肿瘤中染色体臂 6q27 上的 D6S193 (62%) 等位基因丢失频率较高，并绘制了 D6S297 和 D6S264 (3 cM) 之间等位基因丢失的最小区域。我们分离并绘制了包含 D6S193 和 D6S297 的单个非嵌合 YAC（17IA12，260 - 280 kb）。使用 PAC 和 BAC 建立了进一步扩展的细菌重叠群（D6S264 和 D6S149 之间），并建立了转录图谱。我们已经将 6 个新标记映射到 YAC；其中三个是 EST（WI-15078、WI-8751 和 TCP10）。我们分离了 EST WI-15078 的 3 个 cDNA 克隆，其中一个克隆包含完整的开放阅读框。该序列显示出与核糖核酸酶家族新成员的同源性。另外两个克隆是这个新基因的剪接变体。该基因在正常组织中普遍表达。根据 Northern 分析，它在 4/8 卵巢癌细胞系中表达。该基因编码 40 kDa 的蛋白质。对所有八种卵巢癌细胞系中的基因进行直接测序，未发现任何突变。通过将全长基因转染到卵巢癌细胞系中进行克隆形成测定，没有观察到生长抑制。
We have previously shown a high frequency of allele loss at D6S193 (62%) on chromosomal arm 6q27 in ovarian tumours and mapped the minimal region of allele loss between D6S297 and D6S264 (3 cM). We isolated and mapped a single non-chimaeric YAC (17IA12, 260 - 280 kb) containing D6S193 and D6S297. A further extended bacterial contig (between D6S264 and D6S149) has been established using PACs and BACs and a transcript map has been established. We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP10). We have isolated three cDNA clones of EST WI-15078 and one clone contains a complete open reading frame. The sequence shows homology to a new member of the ribonuclease family. The other two clones are splice variants of this new gene. The gene is expressed ubiquitously in normal tissues. It is expressed in 4/8 ovarian cancer cell lines by Northern analysis. The gene encodes for a 40 kDa protein. Direct sequencing of the gene in all the eight ovarian cancer cell lines did not identify any mutations. Clonogenic assays were performed by transfecting the full-length gene in to ovarian cancer cell lines and no suppression of growth was observed.