The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters.
The human initiator is a distinct and abundant element that is precisely positioned in focused core promoters.
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DOI：
10.1101/gad.293837.116
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发表时间：
2017-01-01
影响因子：
10.5
通讯作者：
Kadonaga JT
中科院分区：
文献类型：
--
作者：
Vo Ngoc L;Cassidy CJ;Huang CY;Duttke SH;Kadonaga JT
Vo ngoc等人表明，人类启动子在聚焦启动子上具有BBCA+1BW的共识，其中转录在单个位点或狭窄的位点簇上启动。DNA序列信号在核心启动子中，如启动子（Inr），由RNA聚合酶II直接转录起始。在这里，我们表明人类Inr在聚焦启动子上具有BBCA+1BW的共识，其中转录在单个位点或狭窄的位点簇上启动。对7678个集中转录起始位点的分析显示，40%的转录起始位点与Inr完全匹配，16%的转录起始位点在CA+1核心之外有一个不匹配。TATA-like序列在Inr启动子中代表性不足。这一共识是人类指定转录起始的DNA序列规则的关键组成部分。
Vo ngoc et al. show that the human initiator has the consensus of BBCA+1BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. DNA sequence signals in the core promoter, such as the initiator (Inr), direct transcription initiation by RNA polymerase II. Here we show that the human Inr has the consensus of BBCA+1BW at focused promoters in which transcription initiates at a single site or a narrow cluster of sites. The analysis of 7678 focused transcription start sites revealed 40% with a perfect match to the Inr and 16% with a single mismatch outside of the CA+1 core. TATA-like sequences are underrepresented in Inr promoters. This consensus is a key component of the DNA sequence rules that specify transcription initiation in humans.