Mannose receptor and its putative ligands in normal murine lymphoid and nonlymphoid organs: In situ expression of mannose receptor by selected macrophages, endothelial cells, perivascular microglia, and mesangial cells, but not dendritic cells.
Mannose receptor and its putative ligands in normal murine lymphoid and nonlymphoid organs: In situ expression of mannose receptor by selected macrophages, endothelial cells, perivascular microglia, and mesangial cells, but not dendritic cells.
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DOI：
10.1084/jem.189.12.1961
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发表时间：
1999-06-21
期刊：
影响因子：
--
通讯作者：
Gordon S
中科院分区：
文献类型：
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作者：
Linehan SA;Martínez-Pomares L;Stahl PD;Gordon S
甘露糖受体（MR）在巨噬细胞（Mφ）对微生物的吞噬以及对宿主来源糖蛋白的内吞清除中具有确定的作用，并且最近在体外被认为与树突状细胞（DCs）的抗原捕获有关。MR是同源蛋白家族的创始成员，其识别特性因起源组织而异。鉴于这种异质性以及我们最近在小鼠血清中发现的一种可溶性MR形式，我们研究了正常小鼠组织中MR mRNA的合成位点和MR蛋白的表达。我们证明合成和表达发生在相同位点，并且成熟的Mφ和内皮细胞在MR表达方面是异质的，还描述了血管周围小胶质细胞和肾小球系膜细胞上的MR。然而，在原位的DCs上，或者在边缘区或被膜下窦Mφ上均未检测到MR，而这两者都具有类似MR的结合活性。我们还比较了MR的表达与一种含有MR富含半胱氨酸结构域的重组探针的结合情况。我们表明MR及其假定的配体在淋巴器官内的非重叠位点表达，这与可溶性MR的转运功能是一致的。因此，除了内吞和吞噬作用外，MR可能在淋巴器官内的抗原识别和转运中发挥重要作用。
The mannose receptor (MR) has established roles in macrophage (Mφ) phagocytosis of microorganisms and endocytic clearance of host-derived glycoproteins, and has recently been implicated in antigen capture by dendritic cells (DCs) in vitro. MR is the founder member of a family of homologous proteins, and its recognition properties differ according to its tissue of origin. Given this heterogeneity and our recent discovery of a soluble form of MR in mouse serum, we studied the sites of synthesis of MR mRNA and expression of MR protein in normal mouse tissues. We demonstrate that synthesis and expression occur at identical sites, and that mature Mφ and endothelium are heterogeneous with respect to MR expression, additionally describing MR on perivascular microglia and glomerular mesangial cells. However, MR was not detected on DCs in situ, or on marginal zone or subcapsular sinus Mφ, both of which have MR-like binding activities. We also compared expression of MR to the binding of a recombinant probe containing the cysteine-rich domain of MR. We show that MR and its putative ligand(s) are expressed at nonoverlapping sites within lymphoid organs, consistent with a transfer function for soluble MR. Therefore, in addition to endocytic and phagocytic roles, MR may play an important role in antigen recognition and transport within lymphoid organs.