Modification of rhizobacterial populations by engineering bacterium utilization of a novel plant-produced resource
Modification of rhizobacterial populations by engineering bacterium utilization of a novel plant-produced resource
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DOI：
10.1038/nbt0497-363
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发表时间：
1997-04
影响因子：
46.9
通讯作者：
M. Savka;S. Farrand
中科院分区：
文献类型：
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作者：
M. Savka;S. Farrand
当与其他根际微生物竞争时，分解代谢植物产生的不同营养物质的能力可能是细菌成功定殖该宿主的因素。我们通过研究由转基因植物产生的新型基质对近等基因细菌根定植的影响来验证这一假设，不同之处仅在于它们利用资源的能力。当单独接种时，两种细菌以相同的动力学和不可区分的水平定殖于正常和转基因植物的根。当这两种细菌共接种时，在资源生产植物的根部，分解代谢菌的种群密度显著高于非分解代谢菌。在正常植物的根上没有观察到这种优势。这些结果支持了这样的理论，即植物宿主产生和渗出的资源可以赋予使用基质的微生物选择优势。
The ability to catabolize distinct nutrients produced by a plant may be a factor in the successful colonization of that host by a bacterium when in competition with other rhizosphere microorganisms. We tested this hypothesis by examining the influence of a novel substrate produced by a transgenic plant on root colonization by near-isogenic bacteria, differing only in their ability to use the resource. When inoculated alone, both bacteria colonized the roots of the normal and transgenic plants with equal kinetics and to indistinguishable levels. When the two bacteria were coinoculated, the catabolizer reached a population density significantly higher than that of the noncatabolizer on the roots of the resource-producing plant. No such advantage was observed on the roots of normal plants. These results support the theory that resources produced and exuded by a plant host can confer a selective advantage to microorganisms that use the substrate.