Identification of short-lived long non-coding RNAs as surrogate indicators for chemical stress response
Identification of short-lived long non-coding RNAs as surrogate indicators for chemical stress response
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DOI：
10.1016/j.bbrc.2013.09.006
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发表时间：
2013-10-04
影响因子：
3.1
通讯作者：
Torimura, Masaki
中科院分区：
文献类型：
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作者：
Tani, Hidenori;Torimura, Masaki
人体细胞中的非生物和生物应激源通常是环境因素突然和/或频繁变化的结果。对应激的分子反应涉及基因表达的精细调节，具有稳态、生态和进化的重要性。尽管人们的注意力主要集中在信号通路和蛋白质网络上，但长链非编码 RNA (ncRNA) 越来越多地参与与细胞应激反应相关的分子机制。我们鉴定了六种新型短寿命长 ncRNA（MIR22HG、GABPB-AS1、LINC00152、IDI2-AS1、SNHG15 和 FLJ33630），它们对 HeLa Tet-off 细胞中的化学应激源（顺铂、放线菌酮和氧化汞 (II)）做出反应。我们的结果表明，短寿命的长 ncRNA 对一般和特定的化学应激源有反应。由于对化学应激源的响应延长的衰减率和 RNA 降解途径的中断，短寿命的长 ncRNA 的表达水平升高。我们认为这些长 ncRNA 有可能成为细胞应激反应的替代指标。 (C) 2013 Elsevier Inc. 保留所有权利。
Abiotic and biotic stressors in human cells are often a result of sudden and/or frequent changes in environmental factors. The molecular response to stress involves elaborate modulation of gene expression and is of homeostatic, ecological, and evolutionary importance. Although attention has primarily focused on signaling pathways and protein networks, long non-coding RNAs (ncRNAs) are increasingly involved in the molecular mechanisms associated with responses to cellular stresses. We identified six novel short-lived long ncRNAs (MIR22HG, GABPB-AS1, LINC00152, IDI2-AS1, SNHG15, and FLJ33630) that responded to chemical stressors (cisplatin, cycloheximide, and mercury (II) oxide) in HeLa Tet-off cells. Our results indicate that short-lived long ncRNAs respond to general and specific chemical stressors. The expression levels of the short-lived long ncRNAs were elevated because of prolonged decay rates in response to chemical stressors and interruption of RNA degradation pathways. We propose that these long ncRNAs have the potential to be surrogate indicators of cellular stress responses. (C) 2013 Elsevier Inc. All rights reserved.