Solution Structure of Human Pex5·Pex14·PTS1 Protein Complexes Obtained by Small Angle X-ray Scattering
Solution Structure of Human Pex5·Pex14·PTS1 Protein Complexes Obtained by Small Angle X-ray Scattering
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小角X射线散射法获得人Pex5·Pex14·PTS1蛋白复合物的溶液结构
DOI：
10.1074/jbc.m109.002311
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
D. Svergun
中科院分区：
文献类型：
--
作者：
K. Shiozawa;P. Konarev;Christian Neufeld;M. Wilmanns;D. Svergun
Pex 5 p受体识别新合成的过氧化物酶体基质蛋白，其具有针对过氧化物酶体的C-末端过氧化物酶体靶向信号。在与膜上的蛋白质复合物对接后，这些蛋白质跨膜易位。对接机制仍不清楚，因为没有多组分对接复合物的结构数据。由于货物装载的Pex 5 p受体和过氧化物酶体膜蛋白Pex 14 p的相互作用是必不可少的主要对接步骤，我们已经研究了这些复合物的小角X射线散射和静态光散射的溶液结构。滴定研究得出Pex 5 p·Pex 14 p复合物的化学计量比为1：6，并且从X射线散射数据重建了低分辨率结构模型。游离的全长人Pex 5 p在溶液中是单体的，具有伸长的、部分未折叠的N-末端结构域。该复合物的模型表明，在货物和Pex 14 p的存在下，Pex 5 p的N末端保持延伸，后者蛋白质与Pex 5 p部分显著地混合。这些结果表明，Pex 5 p的扩展结构可能在与其他底物如脂质和膜蛋白的相互作用中发挥作用，在形成功能性多蛋白复合物。
The Pex5p receptor recognizes newly synthesized peroxisomal matrix proteins which have a C-terminal peroxisomal targeting signal to the peroxisome. After docking to protein complexes on the membrane, these proteins are translocated across the membrane. The docking mechanism remains unclear, as no structural data on the multicomponent docking complex are available. As the interaction of the cargo-loaded Pex5p receptor and the peroxisomal membrane protein Pex14p is the essential primary docking step, we have investigated the solution structure of these complexes by small angle x-ray scattering and static light scattering. Titration studies yielded a 1:6 stoichiometry for the Pex5p·Pex14p complex, and low resolution structural models were reconstructed from the x-ray scattering data. The free full-length human Pex5p is monomeric in solution, with an elongated, partially unfolded N-terminal domain. The model of the complex reveals that the N terminus of Pex5p remains extended in the presence of cargo and Pex14p, the latter proteins being significantly intermingled with the Pex5p moiety. These results suggest that the extended structure of Pex5p may play a role in interactions with other substrates such as lipids and membrane proteins during the formation of functional multiprotein complexes.
影响因子：
16
作者：
Stanley, Will A.;Filipp, Fabian V.;Wilmanns, Matthias
通讯作者：
Wilmanns, Matthias
DOI：
10.1073/pnas.160259697
发表时间：
2000-08-01
影响因子：
11.1
作者：
Shoemaker, BA;Portman, JJ;Wolynes, PG
通讯作者：
Wolynes, PG