Molecular characteristics of human coxsackievirus B1 infection in Korea, 2008-2009
Molecular characteristics of human coxsackievirus B1 infection in Korea, 2008-2009
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DOI：
10.1002/jmv.23359
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发表时间：
2013-01-01
影响因子：
12.7
通讯作者：
Cheon, Doo-Sung
中科院分区：
文献类型：
--
作者：
Kim, HyeJin;Kang, Byunghak;Cheon, Doo-Sung
本研究分析了20082009年度韩国新生儿重症病例和死亡病例中柯萨奇病毒B1感染的流行病学和分子特征。通过全国性的监测项目，收集了104名感染CVB1的患者的标本。从标本中检测肠道病毒(EVS)的方法是在5‘-非编码区(NCR)进行诊断实时聚合酶链式反应(RT-PCR)。用半套式聚合酶链式反应扩增VP1区序列，并与Genbank中注册的参考菌株进行序列比较。男女比例约为5：4。CVB1感染者的主要临床表现为无菌性脑膜炎(55.8%)。其他临床症状为疱疹或手足口病(22.1%)和新生儿败血症(7.7%)。CVB1分离株的序列被划分为四个遗传簇(AD)，簇之间至少有15%的多样性。2008年至2009年韩国几乎所有的CVB1分离株都属于D群(除2例病例外)。韩国CVB1株与美国株的同源性也在93%以上。20082009期间发现的韩国CVB1型毒株有可能来源于20062008期间发现的美国毒株。在韩国发现的CVB1表明，电动汽车有可能以一种不可预测的方式导致严重疾病。J.Med.维罗尔。2012年，85：110115。(C)2012年威利期刊公司。
This study was performed to analyze epidemiological and molecular characteristics of coxsakievirus (CV) B1 infection associated with severe neonatal illness cases and death in Korea during 20082009. Through a nationwide surveillance program, specimens were collected from 104 patients infected with CVB1. The detection of enteroviruses (EVs) from specimens was subjected to a diagnostic real-time polymerase chain reaction (RT-PCR) in the 5'-non-coding region (NCR). A semi-nested PCR was conducted to amplify sequences from the VP1 region and sequence comparison was performed with reference strains registered in Genbank. Male-to-female ratio confirmed approximately 5:4. The major clinical manifestation of patients infected with CVB1 was aseptic meningitis (55.8%). The other clinical symptoms were herpangina or handfootmouth disease (22.1%) and neonatal sepsis (7.7%). The sequences of CVB1 isolates were divided into four genetic clusters (AD) with at least 15% diversity between the clusters. Almost all the CVB1 isolates in Korea from 2008 to 2009 were in cluster D (except for 2 cases). The homology relationship was also similar between the Korean CVB1 strains and US strain (above 93%). It is possible that Korean CVB1 isolates found during 20082009 originated from the US strains found during 20062008. The identification of CVB1 in South Korea shows the potential of EVs to cause serious disease in an unpredictable fashion. J. Med. Virol. 85:110115, 2012. (c) 2012 Wiley Periodicals, Inc.